HUBEI AGRICULTURAL SCIENCES ›› 2019, Vol. 58 ›› Issue (6): 136-139.doi: 10.14088/j.cnki.issn0439-8114.2019.06.031

• Biological Engineering • Previous Articles     Next Articles

Studies on tissue culture and plant regeneration technology of Cerasus humilis

LIANG Wei-ling, CHEN Cui-guo, MENG Fan-yi, ZHAO Min, SUN Hua-ran, LIU Yan-fen   

  1. College of Landscape and Ecology,Hebei University of Engineering,Handan 056021,Hebei,China
  • Received:2018-06-20 Online:2019-03-25 Published:2019-12-03

Abstract: In order to establish the technical system of in vitro and rapid propagation of Cerasus humilis,the culture conditions of the first generation culture, the proliferation culture and the rooting culture were studied. The semi-lignified stem segments were used as explants. The results showed that the optimum medium of the first generation medium was MS+0.5 mg/L 6-BA+0.1 mg/L IBA,the optimum proliferation medium was MS+0.5 mg/L 6-BA+0.5 mg/L KT+0.05 mg/L IBA+300 mg/L LH, and the optimum proliferation medium was 1/2 MS+0.2 mg/L IBA+0.5 mg/L IAA.

Key words: Cerasus humilis, in vitro culture, rapid propagation

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