Optimization of complex enzyme extracted process of polysaccharide from Thamnolia spp.

doi:10.14088/j.cnki.issn0439-8114.2019.17.028

Abstract

Abstract: Based on single factor experiment, the optimum conditions for the complex enzyme extraction of polysaccharides from the ethnodrug Thamnolia spp. was determined by an orthogonal experiments and analysis of variance taking extraction rate as index. Then the ability of polysaccharides to scavenge DPPH and nitrite was determined in vitro too. The results as followthe extraction rate of Thamnolia spp. polysaccharides was the highest in the case of complex enzymes of cellulase and papain （mass ratio 1∶4）, the enzymolysis temperature at 50 ℃, the enzymolysis time in 1.0 h, pH value at 5.0, and the enzyme concentration in 3.0%. And in these case, the total sugar yield is 10.8%. The Thamnolia spp. polysaccharide extracted by this condition had a certain scavenging effect on nitrite and DPPH free radicals, and showed a dose-effect relationship. When Thamnolia spp. polysaccharide concentration was 2.5 mg/mL, the DPPH radical scavenging rate was 98%. When the polysaccharide concentration was 2.0 mg/mL, the scavenging rate of sodium nitrite was 19.8%. The results showed that the extraction of polysaccharide from Thamnolia spp. by complex enzyme method can significantly increase the extraction rate of polysaccharides. And the polysaccharides extracted from this technology from Thamnolia spp. had good DPPH free radical scavenging ability.

Key words: complex enzymes method, Thamnolia spp., polysaccharides, extraction, orthogonal experiment

CLC Number:

QI Wen-lu, XIAO Cong, ZHAO Yan, LI Xue-ying, YANG Qing-song. Optimization of complex enzyme extracted process of polysaccharide from Thamnolia spp.[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(17): 104-108.

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