Optimization of extraction process and determination of total flavonoids in Hypericum japonicum Thumb.

doi:10.14088/j.cnki.issn0439-8114.2021.03.022

Abstract

Abstract: The total flavonoids of Hypericum japonicum Thumb. was extracted by reflux extraction method. The effects of extraction method, extraction solvent concentration, extraction volume, and extraction time on the total flavonoid content of medicinal materials were investigated by single factor test. Orthogonal experiments were used to optimize the extraction process conditions and screen the best extraction technology of total flavonoids from Hypericum japonicum Thumb.. The results showed that the optimal extraction process of total flavonoids from Hypericum japonicum Thumb. was 75% ethanol, the material-liquid ratio was 1∶30（g/mL）, and the reflux extraction time was 1.0 h. This method in this experiment has good repeatability, and the method is stable and feasible,and can effectively determine the content of total flavonoids in Hypericum japonicum Thumb., and provide a certain scientific basis for the study of the quality standard of this medicinal material.

Key words: Hypericum japonicum Thumb., total flavonoids, extraction technology, UV Vis spectrophotometry

CLC Number:

R284.2

WU Bing, MO Ting-ting, LU Mei-yuan, ZENG Chao, ZHAO Feng-xian. Optimization of extraction process and determination of total flavonoids in Hypericum japonicum Thumb.[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 116-119.

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