Study on extraction technology and content determination of total flavonoids from Chinese medicine Argyreia aceta Lour.

doi:10.14088/j.cnki.issn0439-8114.2021.03.021

Abstract

Abstract: The total flavonoids of Argyreia aceta Lour. were extracted by reflux extraction method. The effects of extraction method, extraction solvent concentration, extraction volume and extraction time on the total flavonoids content of the medicinal materials were investigated by single factor experiment. The orthogonal test was used to optimize the extraction conditions. The best extraction process for a total flavonoid of Argyreia aceta Lour. was screened out. The results showed that the optimal extraction process of a total flavonoid of Argyreia aceta Lour. was 75% methanol, the ratio of material to liquid was 1∶30, and the reflux extraction time was 1.0 h. This method repeatability was good, the method was stable,and can effectively determine the content of total flavonoids in Argyreia aceta Lour. medicinal material, and provide a scientific basis for the quality standard research of this medicinal material.

Key words: Argyreia acuta Lour., total flavonoids, extraction process, UV-visible spectrophotometry

CLC Number:

R284.2

LIU Rui, MA Xiu-mei, ZHOU Jin-gen, MA Jia-bao, WEI Li-fu, CHEN Sheng-bin, ZHAO Feng-xian, CHEN Hong-xia. Study on extraction technology and content determination of total flavonoids from Chinese medicine Argyreia aceta Lour.[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(3): 112-115.

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