Abstract: The extraction conditions of total flavonoids from Cnidium monnieri （L.） Cuss.were optimized by extraction method combined with single factor and response surface method, and the insecticidal and antibacterial activities were tested. The results showed that the optimum extraction conditions were as follows： The Cnidium monnieri powder 10 g, the concentration of ethanol was 64%, the extraction time was 23 min, the solvent volume was 85 mL, and the measured yield of flavonoids was 7.83%. In the insecticidal activity test, the 48 h corrected mortality of 1.0 mg/mL Cnidium monnieri extract against Hyalopterus amygdali and Tetranychina harti was greater than 50%, and the corrected mortality of the two test insects was significantly different, with LC₅₀ of 0.041 and 0.086 mg/mL, respectively. The antibacterial activity showed that the extract of Cnidium monnieri had the best inhibitory activity against Botrytis cinerea, and the EC₅₀ was 21.29 mg/L. It also had strong antibacterial activity against Valsa mali and Sclerotinia sclerotiorum.

Key words: Cnidium monnieri （L.） Cuss., total flavonoids, response surface method, insecticidal activity, antifungal activity