Effects of different fertilization managements on tea garden soil bacterial diversity

doi:10.14088/j.cnki.issn0439-8114.2019.22.015

Abstract

Abstract: A field trial was conducted in tea plantation, nine different soil samples were collected, including rapeseed cake fertilizer and tea formula fertilizer（YSJF1）, urea-formaldehyde slow released fertilizer（YSJF2）, tea formula fertilizer（YSJF3）, animal manure and tea formula fertilizer（YSJF4）, rapeseed cake fertilizer and urea-formaldehyde slow released fertilizer（YSJF5）, coated controlled release fertilizer（YSJF6）, tea compound fertilizer（YSJF7）,no fertilization（YSJF8）, and the chemical fertilizer（YSJF9）. Using Ion S5^TMXL high-throughput sequencing technology, targeting the 16S rRNA gene, the effects of different fertilization patterns on the number, diversity and community structure of soil bacteria in tea plantation, and the correlation between the number, diversity and soil physical and chemical properties was analyzed. At the phylum level, forty-six phyla were obtained in all the treatments, among which Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Firmicutes, Thaumarchaeota and Bacteroidetes were the common dominant bacteria, accounting for 87.08%~96.76% of the total reads. There were significant differences in relative abundance of Proteobacteria, Acidobacteria, Actinobacteria and Chloroflexibetween among different treatments. The index of Ace, Chao1, Shannon and Simpson were 656.34~962.36, 655.79~1 040.41, 5.22~6.54 and 0.925 0~0.969 3, respectively. Correlation analysis and redundancy analysis showed that soil pH, organic matter content and alkali-hydrolyzed nitrogen were correlated with bacterial abundance, alpha diversity index and bacterial community structure, and soil pH was an important environmental factor affecting bacterial community.

Key words: tea garden, fertilization patterns, bacterial community diversity, 16S rRNA

CLC Number:

S154.3

MAO Ying-xin, HUANG Dan-juan, WANG Hong-juan, TAN Rong-rong, CHEN Xun, WANG You-ping. Effects of different fertilization managements on tea garden soil bacterial diversity[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(22): 65-70.

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