Abstract: To provide a theoretical basis for the utilization of bacterial chaff to make fertilizer for agricultural use and realize the reuse of waste resources, the nutrient composition and heavy metals content of spent mushroom substrate of Pleurotus eryngii Pholiotanameko fermented fertilizer was analyzed, and the influence of different fertilizers on the growth of cash crops was evaluated. The results showed that the heavy metal content of spent mushroom substrate conformed to the relevant requirements of industry standard NY 525—2012. The crude protein and crude fiber content decreased significantly after fermentation, while the content of amino acid and organic matter were increased greatly. Compared with the blank control, the mixed fertilizer were applied to cash crops ginger （Alpinia coriandriodora D. Fang）, corn （Zea mays L.） and Chinese cabbage （Brassica chinensis L.） could increase the leaf length, plant height, stem diameter, chlorophyll content, POD activity and soluble protein conten.

Key words: spent mushroom substrate of Pleurotus eryngii Pholiotanameko, fermentation, biological fertilizer, fertilizer efficiency