Abstract: To clarify the uptake and translocation ability of phenazine-l-carboxylic acid-valine（PCA-Val） conjugate in wheat plants, using PCA-Val as a supplied test fungicides, the transportation and the contents of PCA-Val in leaves and roots of wheat plants were determined by ultra performance liquid chromatography-tandem mass spectrometry method after it was applied to leaves. The results showed that, for wheat plants at the tillering stage, when the concentration of the tested agents was 200 μmol/L, the content of the compounds could be detected in the roots of wheat plants, and the content of PCA-L-Val in the roots reached its peak of 15.50 μmol/kg after treatment for 18 h, and that of PCA-D-Val reached the peak at the same time, which was 11.37 μmol/kg. In addition, the conjugates could also be detected in the tiller leaves of wheat, and the content of PCA-L-Val in the tiller reached its peak of 8.74 μmol/kg after treatment for 3 h, while PCA-D-Val reached its peak of 4.54 μmol/kg at the same time. Study proves that, the PCA-valine conjugates has phloem mobility in wheat plants,and also has the particular systemicity from the main stem to the tillers, the systemic mobility of valine conjugates in L configuration were significantly higher than those in D configuration.

Key words: phenazine-l-carboxylic acid -valine conjugate, wheat（Triticum aestivum L.） plants, uptake and translocation