Abstract: A strain of Talaromyces wortmannii was isolated LGT-4 was activated and inoculated on the modified Martin's medium for fermentation. The compounds were separated and purified by chromatography. The inhibitory activities of acetylcholinesterase and phosphatidylinositol 3-kinase of the monomers were determined by enzyme labeling method and in vitro kinase detection kit, and the biological activities of secondary metabolites were studied. The results showed that ten known compounds were isolated and identified as Cyclo-（Pro-Ile） （1）、Cyclo（L-Tyr-L-Leu） （2）、Cyclo-（L-Pro-L-Phe） （3）、Aspergillumarin B （4）、Deacetylisowortmin （5）、Chaetominine （6）、（E）-2-（hydroxymethyl）-3-（2-hydroxypent-3-enyl）phenol （7）、N-（2-Phenylethyl）acetamide （8）、4-hydroxynaphthalide （9）、1,2-Benzenedicarboxylic acid,dibuty ester （10）. Cyclo-（Pro-Ile） （1）shoewed potent anti-acetylcholinesterase activity with IC₅₀ value ??of 11.76 μmol/L. None of the 10 compounds showed significant activity against phosphatidylinositol 3-kinase. Compounds 1,2,3,6,8 were first isolated from this fungus.

Key words: Talaromyces wortmannii LGT-4, secondary metabolites, acetylcholinesterase inhibitors, phosphatidylinositol 3-kinase inhibitors