Bacterial diversity effected by intercropping peach trees in pomegranate wilt disease soil

doi:10.14088/j.cnki.issn0439-8114.2021.14.006

Abstract

Abstract: High-throughput sequencing was used to study the rhizosphere soil bacteria diversity of healthy pomegranate rhizosphere soil（MZ）, wilt disease pomegranate rhizosphere soil （MZD） and wilt disease pomegranate soil intercropping peach trees（MZP） in Mengzi county of Yunnan province, and the bacteria diversity differences of three kinds rhizosphere soil were analyzed to explore the effect of intercropping peach trees on soil bacterial diversity of pomegranate wilt. The results showed that the OTUs numbers obtained from MZD、MZ、MZP were 1 727,1 662,1 510, respectively, PCA analysis showed that the bacterial community composition of rhizosphere soil MZP was significantly different from that of rhizosphere soil samples MZD. Intercropping peach trees can significantly change the bacteria composition of wilt disease pomegranate rhizosphere soil. At genus level, 16 bacteria genus such as Enterococcus, Cellulosimicrobium, Actinomycetospora, Rhodoplanesa et al were significant difference between pomegranate wilt disease soil intercropping peach trees and pomegranate wilt disease soil rhizosphere. Intercropping peach trees can change the rhizosphere bacterial diversity and community composition of wilt pomegranate, thus improving the soil microecological balance, it had positive effect on perennial pomegranate orchard to repair continuous cropping obstacle.

Key words: pomegranate wilt, intercropping, peach tree, bacterial diversity

CLC Number:

S432.2⁺5

ZHOU Yin-li, XIONG Lang, CHEN Yong-ling, PENG Meng-lin, YANG Wei, HU Xian-qi. Bacterial diversity effected by intercropping peach trees in pomegranate wilt disease soil[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(14): 37-40.

References 15

[1]	代真林,汪娅婷,姚秀英,等.玉米大豆间作模式对玉米根际土壤微生物群落特征、玉米产量及病害的影响[J].云南农业大学学报(自然科学),2020,35(5):756-764.
[2]	覃潇敏,郑毅,汤利,等.玉米与马铃薯间作对根际微生物群落结构和多样性的影响[J].作物学报,2015, 41(6):919-928.
[3]	高圣超,关大伟,马鸣超,等.不同大豆种植制度对土壤质量的影响研究进展[J].大豆科技,2017(3):21-29.
[4]	付学鹏,吴凤芝,吴瑕,等.间套作改善作物矿质营养的机理研究进展[J].植物营养与肥料学报,2016, 22(2):525-535.
[5]	李章辉. 花生连作田中土壤微生物群落数量变化分析[J].河南农业,2020(31):15-16.
[6]	许振羽,李学鹏,杜宇,等.桑树根际土壤微生物对间作和施氮的响应[J].应用生态学报,2019,30(6):1983-1992.
[7]	ISHAQ S L, JOHNSON S P, MILLER Z J, et al.Impact of cropping systems, soil inoculum, and plant species identity on soil bacterial community structure[J]. Microbial ecology,2017,73(2):417-434.
[8]	何平,余爽,巫登峰,等.四川攀西地区石榴枯萎病的发生危害及防控建议[J].中国热带农业,2017(5):22-23,47.
[9]	周银丽,袁绍杰,潘云梅,等.放线菌JS2对石榴枯萎病菌及南方根结线虫的毒力研究[J].江西农业大学学报,2016,38(2):268-274.
[10]	周银丽,郭建伟,杨伟,等.石榴枯萎病菌拮抗菌A4的筛选鉴定及定植能力研究[J].江西农业大学学报,2018,40(1):168-173.
[11]	AVERSHINA E,FRISLI T,RUDI K.De novo semi-alignment of 16S rRNA gene sequences for deep phylogenetic characterization of next generation sequencing data[J].Microbes and environments,2013,28(2):211-216.
[12]	杨广容,马燕,蒋宾,等.基于16S rDNA测序对茶园土壤细菌群落多样性的研究[J].生态学报,2019,39(22):8452-8461.
[13]	徐丽霞,何永吉.基于16S rDNA高通量测序的玉米根际与非根际土壤细菌多样性比较[J].山西农业科学, 2019,47(7):1212-1216.
[14]	邓晓,李勤奋,武春媛,等.健康香蕉(Musa paradisiaca)植株与枯萎病患病植株根区土壤细菌多样性的比较研究[J].生态环境学报,2015(3):402-408.
[15]	马云艳,王东胜,李玉龙,等.辣椒疫病病株与健株根区土壤微生态研究[J].西北农业学报,2015,24(4): 129-137.