Study on the effect of carboxymethylation of Siraitia grosvenorii polysaccharide on its antioxidant

doi:10.14088/j.cnki.issn0439-8114.2021.15.021

Abstract

Abstract: In order to study the structure and antioxidant properties of Siraitia grosvenorii polysaccharide and its carboxymethylated products, Siraitia grosvenorii polysaccharide was extracted from Siraitia grosvenorii using enzymatic hydrolysis method and modified by carboxymethylation. GC-MS monosaccharide analysis was carried out and Fourier Infrared Spectroscopy. The scavenging ability of polysaccharides of Siraitia grosvenorii and its carboxymethylation products on 1,1-diphenyl-2-trinitrophenylhydrazine （DPPH） and hydroxyl radical （·OH） was also studied. The results showed that the extraction rate of Siraitia grosvenorii polysaccharide was 3.64, and the degree of carboxymethylation substitution was 0.41. GC/MS technology was used to analyze the monosaccharide composition of Siraitia grosvenorii polysaccharides. Combined with the area normalization method, Siraitia grosvenorii polysaccharides were obtained from furanose （3.53%）, arabinose （7.22%）, mannose （20.60%）, glucose （38.03%）, xylose （0.73%）, galactose （28.65%）, galacturonic acid （0.51%）, glucuronic acid （0.74%）. Compared with that before modification, the polysaccharides modified by carboxymethylation had stronger antioxidant properties.

Key words: Siraitia grosvenorii, polysaccharide, carboxymethylation, antioxidant

CLC Number:

TS24

BAI Jia-feng, YAO Yan-chao, ZHENG Yi, LIU Shao-hua, LIU Qi-bin, ZHOU Yi, XING Yu-qing, YANG Wen-jing, XU Chun-ping. Study on the effect of carboxymethylation of Siraitia grosvenorii polysaccharide on its antioxidant[J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(15): 107-111.

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