Analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction of root bark of Acanthopanax trifoliatus

doi:10.14088/j.cnki.issn0439-8114.2023.12.023

Abstract

Abstract: In order to study the analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction from the root bark of Acanthopanax trifoliatus（BLE）, the analgesia and anti-inflammatory effects of BLE were investigated by hot plate pain method, acetic acid writhing method, xylene-induced mouse ear swelling method, carrageenan-induced mouse paw swelling method and prostaglandin E₂（PGE₂） content determination. The antioxidant activities of BLE were evaluated by DPPH free radical method and ABTS free radical method. The results showed that compared with the blank group, the pain threshold of each BLE dose group at 60 min was significantly or extremely significantly increased, compared with before medication, the pain threshold of each dose group at each time point was significantly or extremely significantly increased and the difference in the pain threshold of each dose group and tripterygium glycosides tablet group were not significantly indigenous. Compared with the blank group, the writhing times of mice in each dose group were extremely significantly reduced; each dose group could significantly or extremely significantly reduce the ear swelling induced by xylene and there was no significant difference in ear swelling between each dose group and tripterygium glycosides tablets group; the high-dose group could extramely significantly reduce the paw swelling induced by carrageenan, and compared with the tripterygium glycosides tablets group, the paw swelling degrees in each dose group showed no significant difference. Compared with the blank group, the content of PGE₂ in high-dose and medium-dose groups was extremely significantly decreased. Compared with the tripterygium glycoside tablet group, there was no significant difference in PGE₂ content in high and medium dose groups. The clearance rate of PDDH free radical by BLE was positively correlated with the increase of concentration, with IC₅₀ of 0.029 mg/ml, which was lower than that of the positive control vitamin C （0.04 mg/mL）. The clearance rate of ABTS free radical was positively correlated with the increase of concentration, with IC₅₀ of 0.033 mg/mL, which was lower than that of the positive control vitamin C （0.042 mg/mL）.

Key words: root bark of Acanthopanax trifoliatus, ethyl acetate fraction, analgesia, anti-inflammatory, antioxidation

CLC Number:

R284
R285

LIANG Zi-jiu, JIANG Lin, LIN Qing-hua, LIN Chang-yuan, ZHANG Ren-yi, HUANG Xiao-hong, LAI Xin-hong, XIE Meng-jiao, CHEN Hai-yan, LI Fang-chan. Analgesia, anti-inflammatory and antioxidant activities of ethyl acetate fraction of root bark of Acanthopanax trifoliatus[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 130-134.

References

[1] 国家中医药管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1999.
[2] 修程蕾,胡心怡,屠宇帆,等.五加属植物白簕活性成分及其应用研究进展[J].亚热带植物科学,2016,45(1):90-94.
[3] 王宝庆,郭鑫磊,刘莹,等.白簕化学成分及其药理活性研究进展[J].北方园艺,2018(13):162-168.
[4] 杨慧文,张旭红,陈健媚,等.白簕叶不同极性部位的体外抗氧化活性分析[J].食品研究与开发,2015,36(3):14-18.
[5] 黄俊生. 二氧化碳超临界萃取白簕黄酮类化合物制备潮州菜特色调味品的研究[J].中国调味品,2017,42(5):57-60.
[6] 陈婉晨,陈素娟,陈怡文,等.白簕叶乙醇提取物对杨桃贮藏生理和品质的影响[J].湖北农业科学,2016,55(7):1791-1794.
[7] 广东省食品药品监督管理局.广东省中药材标准[M].广州:广东科技出版社,2011.
[8] 刘婧,刘燕,赵军,等.大戟脂有效部位及大戟二烯醇抗炎镇痛作用研究[J].医药导报,2021,40(12):1665-1670.
[9] 廖娜,黄光伟,唐红艳,等.大血藤醇提物抗炎镇痛和止血活性研究初探[J].广西植物,2021,41(7):1120-1125.
[10] 苟鼎,张腊梅,黄红琴,等.黑果腺花楸不同极性物质抗氧化及抗炎镇痛研究[J].化学试剂,2022,44(1):90-96.
[11] 雷雨恬,黄婷,陈文莉,等.不同等级川白芷有效成分含量及抗炎镇痛作用对比研究[J].中药药理与临床,2021,37(1):105-110.
[12] 刘久石,张小艺,李斌,等.基于谱效关系的藁本类药材抗炎活性成分研究[J].中国中药杂志,2022,47(12):3295-3302.
[13] 杨悦,刘颖,刘晓谦,等.基于超高效液相色谱串联质谱技术的山楂核抗菌、抗氧化药效物质基础研究[J].世界中医药,2021,16(17):2527-2532.
[14] 杨艾华,宋姗姗,王微微.湄潭白茶多糖抗氧化活性及稳定性研究[J].食品科技,2021,46(10):194-199.
[15] 马娇,施志凡,张海芬,等.7种云南可食用昆虫醇提液对ABTS+自由基清除作用[J].化学工程师,2021,35(1):9-12.
[16] 沈校,王振兴,陈丽琼,等.加拿大一枝黄花提取物的抗氧化活性研究[J].广西植物,2018,38(3):299-305.
[17] 刘玉树,刘飞飞,李家光,等.镇痛药不同给药时机对中等面积二度烧伤患者换药镇痛效果的影响[J].天津医药,2021,49(12):1315-1318.
[18] 徐巍华,盛耀华,岑洁.兔深层巩膜切除术后房水前列腺素E₂的定量研究[J].实用医学杂志,2009,25(10):1571-1573.
[19] 罗香怡,高浩溟,姜丰,等.水液相下羟基自由基(水分子簇)诱导脯氨酸分子损伤的机理[J].复旦学报(自然科学版),2022,61(1):104-114.