Optimization of extraction process of flavonoids from Scutellaria baicalensis stem and leaf by response surface methodology and its content determination

doi:10.14088/j.cnki.issn0439-8114.2024.01.024

Abstract

Abstract: The effects of ethanol concentration, solid-liquid ratio, extraction time and extraction temperature on the extraction of flavonoids from Scutellaria baicalensis stem and leaf were investigated by single factor and response surface experiment. On this basis, the contents of flavonoids in 17 Scutellaria baicalensis stems and leaves were determined by HPLC. The results showed that the optimum extraction condition of flavonoids in Scutellaria baicalensis stem and leaves was ethanol concentration of 55%, solid-liquid ratio of 1∶50, extraction time of 60 min and extraction temperature of 50 ℃. Under the optimal extraction process,the extraction amount of flavonoids was 24.43 mg/g. The contents of total flavonoids in Scutellaria baicalensis stem and leaf from different regions were different, ranging from 15.839 mg/g to 26.044 mg/g. The response surface methodology was stable, accurate and reliable to optimize the extraction process of total flavonoids from Scutellaria baicalensis stem and leaf. The established method for the determination of flavonoids content was stable and reliable, which could provide a reference for the quality evaluation and further development of Scutellaria baicalensis stem and leaf.

Key words: Scutellaria baicalensis stem and leaf, flavonoids, extraction process, high-performance liquid chromatography （HPLC）, content determination, response surface methodology

CLC Number:

R284.2

YAN Jie, ZHANG Si-fang, MAO Xian-xian, XU Chen-xin, LIU Bei. Optimization of extraction process of flavonoids from Scutellaria baicalensis stem and leaf by response surface methodology and its content determination[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(1): 131-137.

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