Abstract: To determine the content of Peucedanum praeruptorum Dunn. A and Peucedanum praeruptorum Dunn. B in the medicinal herb Peucedanum praeruptorum Dunn. from different regions and under different planting conditions, and to analyze the factors affecting the quality of the medicinal herb, the high-performance liquid chromatography and a C18 column （4.6 mm×250 mm, 5 μm） were used. The mobile phase was methanol-water （volume ratio of 75∶25）, with a flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength of 321 nm.When the content of Peucedanum praeruptorum Dunn. A and Peucedanum praeruptorum Dunn. B was 5.0~400.0 μg/L and 2.5~20.0 μg/L, respectively,the linear relationship was good;the relative standard deviations of peak areas for Peucedanum praeruptorum Dunn. A and Peucedanum praeruptorum Dunn. B were 0.04% and 0.05%, respectively. Research had shown that at an altitude of 100~800 meters, the content of Peucedanum praeruptorum Dunn. A in Peucedanum praeruptorum Dunn. did not show significant changes with altitude, but all met the requirements of “Pharmacopoeia of the People’s Republic of China（2020 edition）”;the content of Peucedanum praeruptorum Dunn. B showed an overall upward trend with increasing altitude. As the altitude increased, the temperature difference between day and night increased, which was conducive to the enrichment of Peucedanum praeruptorum Dunn. B;compared to the application of nitrogen, phosphorus, and potassium compound fertilizers, animal manure fermentation organic fertilizer could increase the content of two coumarin compounds; the application of trace element fertilizer on the basis of nitrogen, phosphorus and potassium compound fertilizer was beneficial for the enrichment of Peucedanum praeruptorum Dunn. B.

Key words: Peucedanum praeruptorum Dunn., high-performance liquid chromatography, Peucedanum praeruptorum Dunn. A, Peucedanum praeruptorum Dunn. B, coumarin