Identification of drought resistance of polyploid Oryza sativa L. DHF-CBS gene

doi:10.14088/j.cnki.issn0439-8114.2024.09.036

Abstract

Abstract: In order to explore the drought resistance mechanism of DHF-CBS gene regulation in polyploid Oryza sativa L. under drought stress, two varieties of Oryza sativa L. were selected, namely polyploid plant Zhonghua-11 （DHF-CBS gene） and mutant plant UC-32 （non expressing DHF-CBS gene）,the GUS chemical staining results, tissue moisture content, abscisic acid （ABA） content, thiobarbituric acid reactive substance （TBARS） content, glutathione （GSH） content, etc. of two types of Oryza sativa L. were analyzed. The results showed that on the 7th day of drought stress, some leaves of Zhonghua-11 remained green and firm. On the 11th day of drought stress, the entire plant's leaves turned yellow, and some leaves even withered,after observing the phenotype under drought stress, it was found that Zhonghua-11 had stronger drought resistance than UC-32. Analysis of the water content, ABA content, TBARS content, and GSH content of Oryza sativa L. showed that the drought resistance of Zhonghua-11 was better than that of UC-32, indicating that the DHF-CBS gene enhanced the drought resistance of Oryza sativa L..

Key words: polyploid, Oryza sativa L., DHF-CBS gene, drought resistance

CLC Number:

S511

GE Jie, HE Yu-chi, ZHAN Fan, LI Zhi-long. Identification of drought resistance of polyploid Oryza sativa L. DHF-CBS gene[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(9): 216-220.

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