Acute toxicity test of peptides from monkfish meat in mice

doi:10.14088/j.cnki.issn0439-8114.2024.12.025

Abstract

Abstract: To investigate the acute toxicity of monkfish （Lophius litulon） polypeptides （LPs） in mice, eighteen healthy male ICR mice were randomly divided into three groups： control group, LPs low-dose group （2.5 g/kg）, and LPs high-dose group （10 g/kg）. Each mouse was administered with 20 mL/kg of LPs through gavage, and they were observed continuously for 14 days. The initial weight at the start of the experiment, weight on the 7th day, and weight on the 14th day were recorded. Signs of toxicity and mortality were also observed. At the end of the experiment, blood was collected from the mice’s eyeballs to test for liver and kidney function-related indicators. Heart, liver, kidney, spleen, and lungs were collected for external observation, and the tissues were subjected to HE staining for histological analysis. The results showed that no deaths occurred in any of the three groups of mice. Liver and kidney function-related indicators showed no significant differences compared to the control group （P>0.05）. The heart, liver, kidney, spleen, and lungs of mice in the high-dose and low-dose LPs group appeared normal, and microscopic examination of tissues stained with HE revealed clear structures without abnormal changes. Therefore, Lophius litulon polypeptides had no acute toxicity in mice.

Key words: Lophius litulon, polypeptide, mouse, acute toxicity

CLC Number:

TS209

SHEN Lu-jia, SHEN Meng-ran, HE Jia-wei, TANG Jing, ZHANG Xi, YANG Zui-su. Acute toxicity test of peptides from monkfish meat in mice[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(12): 135-138.

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