Preparation and application of polyclonal antibody against tomato chlorosis virus coat protein

doi:10.14088/j.cnki.issn0439-8114.2025.02.031

Abstract

Abstract: In order to establish a rapid detection technology for the tomato chlorosis virus （ToCV）, the coat protein （CP） gene of the virus was cloned by RT-PCR technology, and it was recombinant into a prokaryotic expression vector using Gateway technology. It was then transformed into Escherichia coli BL21 strain for induction of expression. The purified CP protein was used as an antigen to prepare a ToCV CP polyclonal antibody by immunizing Japanese rabbits. Indirect ELISA showed that the titer of the ToCV CP polyclonal antibody was 1∶12 800. Western blot and field experiments had shown that the ToCV CP polyclonal antibody had good specificity and sensitivity. The ToCV CP polyclonal antibody could be used for the detection of ToCV in the field.

Key words: tomato chlorosis virus（ToCV）, coat protein（CP）, prokaryotic expression, polyclonal antibody, preparation

CLC Number:

S432.41

WU Wei-lan, NIE San-mei, ZHANG Kun, LU Zhou, LI Zhan-biao, JI Ying-hua, GUO Ling-fang, ZHANG Song-bai. Preparation and application of polyclonal antibody against tomato chlorosis virus coat protein[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(2): 197-201.

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