Full-length transcriptome sequencing and bioinformatics analysis of the Bombyx mandarina

doi:10.14088/j.cnki.issn0439-8114.2025.06.033

Abstract

Abstract: This study employed second-generation sequencing (Illumina RNA-Seq) to calibrate third-generation sequencing (PacBio ISO-Seq) for conducting full-length transcriptome sequencing of the Bombyx mandarina, aiming to investigate gene expression characteristics during different pupal diapause and deeply explore genomic functional information.A total of 93 616 full-length transcripts were obtained through sequencing and assembly, with a sequence length of 327~33 273 bp, an average length of 2 631 bp, and N50 of 3 204 bp. Integration of annotations from COG, GO, KEGG, KOG, Pfam, Swiss Prot, eggNOG, and NR databases identified 82 796 functionally annotated genes. Analysis of full-length transcripts revealed 17 189 lncRNAs, 87 921 SSR markers, and 49 432 open reading frames (ORFs), with ORF-encoded proteins ranging from 0 to 1 522 amino acids (average 305 aa).Gene expression analysis across diapause stages identified 5 780 differentially expressed genes, including 2 269 with GO annotations and 1 590 with KEGG annotations.

Key words: Bombyx mandarina, full-length transcriptome, sequencing, bioinformatics analysis

CLC Number:

Q966
S881.2

MENG Gang, WANG Rui-xian, CHU Qu, PENG Yun-wu, YANG Jin-hong, CHEN An-li, ZHANG Sheng-yuan, LING Jun. Full-length transcriptome sequencing and bioinformatics analysis of the Bombyx mandarina[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 197-206.

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