Effects of plastic film mulching on physicochemical properties and fungal community structure of tobacco-growing soil during whole growth period

doi:10.14088/j.cnki.issn0439-8114.2023.12.009

Abstract

Abstract: Using white plastic film covering, which was widely used in tobacco production practice, and the appropriate time for film removal treatment as a control, the effects of different colors of plastic film （white plastic film, black plastic film and black and white double color plastic film） covering during the whole growth period on the physicochemical properties and fungal community structure of tobacco planting soil at a higher altitude （1 200 m） were studied, and the relationship between soil physicochemical properties and fungal community structure was explored by correlation and redundancy analysis. The results showed the yield and output value of flue-cured tobacco in the whole growth period were significantly higher than those in the control （P<0.05）, and there were significant differences in soil physical and chemical properties among different treatments （P<0.05）. The main groups of soil fungi in this area were Ascomycota, Basidiomycota and Zygomycota, accounting for more than 83% of the total fungi. The diversity of soil fungi under mulching with different colors during the whole growth period was as follows from high to low： black and white double color film covering the whole growth period, white film covering the whole growth period, control, black film covering the whole growth period. The dominant genera of bacteria in each treatment were significantly different. The relative abundance of Hypocrea, Ceratobasidium, Fusarium and Nectria were closely related to soil nitrogen and phosphorus. macroelements （total nitrogen, alkaline hydrolysis nitrogen, total phosphorus, available phosphorus, total potassium）, medium and trace elements （total sulfur, total copper, available copper, total zinc, available magnesium, total boron） were the leading environmental factors on the community structure of tobacco-growing soil. On the whole, it was feasible to cover the film during whole growth period in the high altitude tobacco area of Yibin City, and the black and white double color film was the optimal choice.

Key words: tobacco, soil, film mulching, fungal community, soil physical and chemical properties, high-throughput sequencing

CLC Number:

S151.9

GUO Chong, LIU Ya-jie, YANG Yi-de, YANG Yang, YANG Guo-wei, XU Yan-ling, CHEN Fo-yuan, HAN Wei, LIU Lei, YAN Min, ZENG Shu-hua. Effects of plastic film mulching on physicochemical properties and fungal community structure of tobacco-growing soil during whole growth period[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(12): 38-45.

References

[1] 陆龙平, 何承刚.不同揭膜时间对烤烟产质量和土壤微生物影响的研究[A].云南省土壤肥料学会第十一届理事会论文集[C]. 昆明:云南省土壤肥料学会, 2013.136-140.
[2] 时向东, 耿伟, 焦枫, 等.不同覆盖方式下烤烟根际土壤微生物数量动态变化[J].华北农学报, 2010,25(3): 221-224.
[3] 樊俊, 谭军, 邓建强, 等.不同地膜的降解性能及对烟株生长和土壤环境的影响[J].中国烟草科学, 2019,40(4): 22-28.
[4] 马海霞, 张丽丽, 孙晓萌, 等.基于宏组学方法认识微生物群落及其功能[J].微生物学通报, 2015, 42(5): 902-912.
[5] 李慧,何晶晶,张颖,等.宏基因组技术在开发未培养环境微生物基因资源中的应用[J].生态学报,2008,28(4):1762-1773.
[6] 李洁,李睿玉,杨红,等.土壤微生物多样性的研究方法[J].山西农业科学, 2016, 44(11): 1738-1742, 1746.
[7] 孔德钧, 潘文杰, 熊晶, 等.地膜覆盖对高海拔地区烤烟产量和品质的影响[J].贵州农业科学, 2011, 39(6): 58-60.
[8] 杨春江, 张晓海, 金鑫, 等.不同揭膜时间对多雨烟区烤烟根系鲜重的影响[J].云南农业大学学报, 2017, 32(5): 838-845.
[9] 李欢, 李晓林, 张俊伶, 等.蚯蚓与丛枝菌根真菌的相互作用及其对植物的影响[J].土壤学报, 2011, 48(4): 847-855.
[10] 郭伟, 耿珍珍, 陈朝, 等.模拟氮沉降增加对长白山红松和水曲柳菌根真菌群落结构及多样性的影响[J].生态环境学报, 2018, 27(1): 10-17.
[11] FERRIS H, TUOMISTO H.Unearthing the role of biological diversity in soil health[J]. Soil biology and biochemistry, 2015, 85: 101-109.
[12] 李朋发, 杨龙, 李桂龙, 等.基于FUNGuild的镰刀菌根腐病发病烟株根际真菌群落研究[J].中国烟草学报, 2019, 25(2): 63-68.
[13] 潘义宏, 蔡宪杰, 周芳芳, 等.不同施肥措施对植烟土壤及云烟105烤烟病害的影响研究[J].江西农业学报, 2020, 32(2): 80-85.
[14] KARLSSON I, FRIBERG H, STEINBERG C, et al.Fungicide effects on fungal community composition in the wheat phyllosphere[J]. PLoS one, 2014, 9(11):16270043.
[15] 鲁如坤. 土壤农业化学分析方法[M]. 北京: 中国农业科学技术出版社, 2000.
[16] LIU G S.Standard method of observation and analysis of the Chinese ecosystem research network-analysis of the soil physicochemical characteristics and delineation of the soil profiles[M].Beijing: Standard press. 1996.
[17] 闫克玉, 赵献章. 烟叶分级[M]. 北京: 中国农业出版社,2003.
[18] CAPORASO J G, KUCZYNSKI J, STOMBAUGH J, et al.2010 QIIME allows analysis of high-throughput community sequencing data[J]. Nature methods, 2010, 7: 335-336.
[19] 郭亮. 地膜覆盖对烤烟的生长发育和产质量影响研究进展[J].安徽农业科学, 2011,39(10):5755-5756,5760.
[20] 李海波. 浅谈地膜覆盖不同揭膜时期对烤烟质量的影响[J].吉林农业, 2012(2): 63-64.
[21] 唐经祥, 孙敬权, 任四海.烤烟地膜覆盖栽培存在的问题及对策[J].烟草科技, 2000(9): 42-44.
[22] 陈军. 地膜覆盖对烤烟生长发育和养分吸收的影响[D].长沙:湖南农业大学, 2003.
[23] DIOSMA G, AULICINO M, CHIDICHIMO H, et al.Effect of tillage and N fertilization on microbial physiological profile of soils cultivated with wheat[J]. Soil & tillage research, 2006,91(1-2): 236-243.
[24] MENDES L W, TSAI S M, NAVARRETE A A, et al.Soil-borne microbiome: Linking diversity to function[J]. Microbial ecology, 2015, 70(1): 255-265.
[25] JAISWAL A K, ELAD Y, PAUDEL I, et al.Linking the belowground microbial composition, diversity an d activity to soil borne disease suppression and growth promotion of tomato amended with biochar[J]. Scientific reports, 2017, 7: 44382.
[26] SHEN G, ZHANG S, LIU X, et al.Soil acidification amendments change the rhizosphere bacterial community of tobacco in a bacterial wilt affected field[J]. Applied microbiology and biotechnology, 2018, 102(22): 9781-9791.
[27] 桑维钧, 祝明亮, 吴兴禄, 等.烟草镰刀菌根腐病研究初报[J].山地农业生物学报, 1998(3): 140-141,145.
[28] 邱睿, 白静科, 李成军, 等.河南烟草镰刀菌的分子鉴定及致病性分析[J].中国烟草学报, 2018, 24(2): 129-134.
[29] 陈懿, 吴春, 李彩斌, 等.炭基肥对植烟黄壤细菌、真菌群落结构和多样性的影响[J].微生物学报, 2020, 60(4): 39-52.
[30] 杨宇虹, 陈冬梅, 晋艳, 等.不同肥料种类对连作烟草根际土壤微生物功能多样性的影响[J].作物学报, 2011, 37(1): 105-111.
[31] ZHANG Y G, CONG J, LU H, et al.Soil bacterial diversity patterns and drivers along an elevational gradient on Shennongjia Mountain[J]. Microbial biotechnology, 2015, 8(4): 739-746.
[32] 张于光, 宿秀江, 丛静, 等.神农架土壤微生物群落的海拔梯度变化[J].林业科学, 2014, 50(9):161-166.
[33] 丁建莉, 姜昕, 马鸣超, 等.长期有机无机肥配施对东北黑土AM真菌群落结构的影响[J].植物营养与肥料学报, 2017, 23(4): 914-923.
[34] 杨超, 刘国顺, 邱立友, 等.不同植烟土壤微生物数量调查研究[J].中国烟草科学, 2007, 28(5): 3l-36.
[35] 李贺,张楠楠, 马琨.宁夏荒漠草原区土地利用方式对AM真菌多样性的影响[J].西北农业学报, 2018, 27(9): 1-8.
[36] 李小林, 李昕竺, 袁红梅, 等.烟草土壤真菌群落结构和多样性及环境因子对其影响[J].四川师范大学学报(自然科学版), 2015,38(1):134-141.
[37] 韦俊,谭小兵,杨焕文,等.烤烟不同套作模式对土壤理化性质和真菌群落结构的影响[J].土壤通报,2017,48(3):631-638.