Complete genome sequencing and phylogenetic analysis of the Wuhan isolate of soybean mosaic virus

doi:10.14088/j.cnki.issn0439-8114.2025.09.036

Abstract

Abstract: Soybean leaves showing mosaic symptoms were collected from Wuhan, and a soybean mosaic virus (SMV) strain, named SMV-WH, was obtained through RT-PCR detection and whole genome amplification. The complete genome of SMV-WH was 9 940 nt in length, with an open reading frame encoding a polyprotein located at 111~9 719 nt. Sequence analysis showed that the whole genome nucleotide sequence identity between SMV-WH and 164 SMV strains from GenBank ranged from 74.88% to 98.36%, and the amino acid sequence identity ranged from 75.15% to 99.09%, indicating that SMV-WH had high genetic diversity. Phylogenetic analysis showed that SMV-WH clustered with Chinese isolates LJZ010, LJZ002, DSMZ PV-0841, DSMZ PV-0938, JSJJ00 and SCSN, and Korean isolate Anseong, and was most closely related to Anseong and SCSN. The E299K mutation in the protein 1（P1） altered the protein kinase C phosphorylation site, while the G593S mutation in the helper component proteinase(HC-Pro) changed the casein kinase Ⅱ phosphorylation site. Three-dimensional structure prediction indicated that these mutations caused changes in protein conformation.

Key words: soybean mosaic virus, Wuhan isolate, whole genome sequencing, phylogenetic analysis

CLC Number:

S435.2

DENG Ke-lin, LU Zi-hua, LI Yan-jiao, YANG Yuan-xiao, LI Chao, CHEN Shui-lian, YANG Hong-li, SHAN Zhi-hui, HAO Qing-nan, CHEN Hai-feng. Complete genome sequencing and phylogenetic analysis of the Wuhan isolate of soybean mosaic virus[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 238-245.

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