Identification of PGAM5 gene core promoter and analysis of transcriptional regulation

doi:10.14088/j.cnki.issn0439-8114.2025.10.025

Abstract

Abstract: To analyze the regulation of intracellular PGAM5 expression after PEDV infection and to refine the mechanism of action of PEDV virus. In this experiment, the expression of PGAM5 after PEDV infection of LLC-PK1 cells were analyzed by qRT-PCR, and screened the core region of the promoter by step-wise method, and screened the transcription factors that bind to the core promoter of the PGAM5 gene by the JASPAR database and preliminarily verified the screened genes by qRT-PCR. The results showed that PGAM5 gene mRNA expression was up-regulated after PEDV infection of LLC-PK1 cells. The core region of PGAM5 promoter was screened to be located between -60 bp and -1 bp. The transcription factors TFAP2A, TCFL5, ZNF354C and ARNT were preliminarily verified to have transcriptional regulation of the PGAM5 promoter by qRT-PCR.

Key words: PEDV, PGAM5, core promoter, transcription factor

CLC Number:

S852.65

LI Ze-ya. Identification of PGAM5 gene core promoter and analysis of transcriptional regulation[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(10): 165-170.

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