Abstract: The potential of Hanseniaspora uvarum aroma-enhancing medium prepared by Hanseniaspora uvarum and tobacco flower buds to improve the quality of solid-state fermented cigar leaves under optimized preparation conditions was explored. After single factor optimization of carbon source (glucose), nitrogen source (isoleucine) concentration and inoculation amount during the preparation of aroma-enhancing medium, it was found that the total aroma reached the highest level when the glucose concentration was 10% of the aroma medium system, the isoleucine concentration was 0.8%, and the inoculation amount was 2.8% of the weight of cigar tobacco leaves. After optimizing the above three conditions by response surface methodology, the total amount of aroma could reach 1 098.70 μg/g. At this time, glucose in the fermentation broth consumed 54.45 g/L, isoleucine decreased by 0.63 g/L, the number of viable bacteria increased by 0.78×10⁸ CFU/mL, and the content of acids and aldehydes in the volatile aroma substances of the fermentation broth increased. This research could provide novel strategies and theoretical support for the development of aroma-enhancing medium for cigars in China.

Key words: Hanseniaspora uvarum, tobacco buds, aroma-enhancing medium, cigar tobacco leaves, response surface optimization