Preliminary Study on Rapid Propagation in Vitro of Three Kinds of Lactuca sativa

doi:10.14088/j.cnki.issn0439-8114.2018.06.028

Abstract

Abstract: In this experiment,three kinds of cotyledons from Lactuca sativa were used as explants to study the effects of different plant growth substances on the induction of adventitious buds and the in vitro regeneration. The results showed that the optimal medium for the induction of adventitious buds from Lactuca sativa L. var. ramosa Hort.,Lactuca sativa L. var. crisha Hort. and Lactuca sativa var capitata L. were MS+0.1 mg/L 6-BA+0.2 mg/L NAA,MS+0.1 mg/L 6-BA+0.1 mg/L NAA,and MS+0.2 mg/L 6-BA+0.1 mg/L NAA,respectively. The optimum rooting medium for the three kinds of Lactuca sativa were 1/2 MS+0.05 mg/L NAA,1/2 MS,and 1/2 MS+0.1 mg/L NAA. By analyzing the factors that affect the establishment of high-frequency regeneration system of three kinds of Lactuca sativa,it laid a foundation for further development of functional vegetables by using genetic engineering technology.

Key words: Lactuca sativa, rapid propagation in vitro, plant growth substance, adventitious buds, rooting, regeneration

CLC Number:

S636.2

LI Mei, LU Yu-jian, BIAN Wen-jing, ZHANG Huan-huan, SHI Dong-li. Preliminary Study on Rapid Propagation in Vitro of Three Kinds of Lactuca sativa[J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(6): 111-115.

References

[1] TACKET C O,MASON H S,LOSONSKY G,et al.Immunogenicity in humans of a recombinant bacterial antigen delivered in transgenic potato[J].Nat Med,1998,4:607-609.
[2] 邓小莉,常景玲.口蹄疫抗原决定簇融合基因转化生菜及表达[J].植物学通报,2007,24(5):609-613.
[3] ARNTZEN C J.High-tech herbal medicine:Plant-based vaccines[J].Nature Biotechnology,1997,15(3):221-222.
[4] TACKET C O,MASON H S.A review of oral vaccination with transgenic vegetables[J].Microbes Infect,1999,1(10):777-783.
[5] 张宝海,韩向阳.开发适宜生食的蔬菜种类和实用方法[J].中国蔬菜,2006(4):36-37.
[6] 邓小莉,常景玲,贺杰,等.口蹄疫抗原决定簇融合基因转化生菜的研究[J].武汉植物学研究,2006,24(5):476-479.
[7] NEGROUK V,EISNER G,LEE H I,et al.Highly efficient transient expression of functional recombinant antibodies in lettuce[J].Plant Science,2005,169:433-438.
[8] LI J,CHEN M,LIU X W,et al.Transient expression of an active human interferon-beta in lettuce[J].Scientia Horticulturae,2007,112:258-265.
[9] BOYHAN D,DANIELL H.Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide[J].Plant Biotechnology Journal,2011,9:585-598.
[10] CUI L J,CHEN Y H,SHEN G A,et al.Expression of bioactive thymosin alpha 1(Tα1) in marker-free transgenic lettuce (Lactuca sativa)[J].Plant Molecular Biology Reporter,2011, 29:466-472.
[11] PNIEWSKI T,KAPUSTA J,BOCIAG P,et al.Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation[J].J Appl Genetics, 2011,52:125-136.
[12] 王兴翠,吕金浮,薛其勤,等.应用TDZ优化生菜再生体系的建立[J].浙江农业学报,2015,27(8):1381-1386.
[13] 陈筑,刘建国,张燕,等.转基因防龋生菜叶片转化体系的优化[J].种子,2012,31(8):5-9.
[14] 李静,许彬,李文露,等.生菜组织培养再生体系的建立[J].农业研究与应用,2013(3):8-10.
[15] 邓莹,罗雯.意大利生菜组织培养体系的建立[J].安徽农业科学,2016,44(2):181-183.
[16] 罗雯,孙东妮,王甜,等.生菜基因转化组织培养受体系统的建立[J].科学技术与工程,2010,10(34):8384-8388.
[17] 朱路英,刘玲,孟祥栋,等.叶用莴苣离体培养和植株再生[J].园艺学报,2002,29(2):181-182.
[18] 乔旭,刘玲,高洋,等.罗马生菜的组织培养及无性系建立[J].现代农业科技,2008(4):5-6,9.