Abstract: To establish an efficient tissue culture system for rapidly propagating, taking young stem section of Ilex crenata ender as explant, different bud-induction medium, proliferation medium, robust seedling medium and rooting medium were conducted. The results showed that the optimal bud-induction medium was MS+ZT 0.5~1.0 mg/L+NAA 0.1 mg/L and the induction rate of axillary bud was about 95%; The optimal medium for proliferation was MS+ZT 0.5 mg/L+NAA 0.2 mg/L,multiplication coefficient was high, seedling growth was strong with thick green; The optimal robust seedling medium was MS+ZT 0.5 mg/L+NAA 0.2 mg/L+sugar 30 g/L, the height of seedling reached 4.9 cm; The optimal rooting medium was 1/2 MS+IBA 0.1 mg/L+NAA 0.5 mg/L+active-carbon 3.0 g/L, and the roots were strong and white in color, the rooting rate was 100%; The survival percentage of plantlets could be up to 95% through transplanted in the substrate containing garden soil, vermiculite and peat （2∶1∶1,V/V/V）.

Key words: Ilex crenata, tissue culture and rapid propagation, induction rate, rooting rate