Abstract: The effects of different plant growth regulators on the induction of tea[Camellia sinensis（L.） O. Ktze.] root and leaf callus in MS medium were investigated by using the germinated roots and leaves of tea seeds as explants under aseptic conditions. The results showed that the optimum medium for callus induction was NAA 1.0 mg/L+6-BA 0.5 mg/L+2,4-D 1.5 mg/L, and the induction rate was 85.1%. NAA 1.0 mg/L+6-BA 0.5 mg/L+2,4-D 2.0 mg/L was the optimal medium for callus induction in young leaves, with an induction rate of 87.0%.

Key words: tea tree[Camellia sinensis （L.） O. Ktze.], callus, tissue culture, induction rate