Study on anti-browning and stem tissue culture of Zanthoxylum nitidum （Roxb.）DC.

doi:10.14088/j.cnki.issn0439-8114.2019.22.044

Abstract

Abstract: Tender stems were used as explants, to discuss the effects of different anti-browning agents on the browning of plantlets in tissue culture of Zanthoxylum nitidum. The results showed that when adding 1.0 g/L PVP in first culture medium, the explants were light browning, The highest budding rate was 62.50%. Three combinations of adding anti-browning agent played a role in inhibiting browning on test tube plantlet,The best result was to add PVP. The browning rate was 11.67% in the presence of PVP was 1.0 g/L, the multiplication coefficient was 5.03,followed by AC,the anti browning effect of Vc was not obvious.

Key words: Zanthoxylum nitidum （Roxb.）DC., stems, tissue culture, test tube plantlet, anti-browning

CLC Number:

S853.75

WEI Ying, YU Li-ying, HUANG Xue-yan, MIAO Jian-hua, WEI Shu-gen, DONG Qing-song, QIN Shuang-shuang, HUANG Bao-you. Study on anti-browning and stem tissue culture of Zanthoxylum nitidum （Roxb.）DC.[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(22): 194-197.

References 15

[1]	温尚开. 两面针的研究概况[J].中草药,1995,26(4):215-217.
[2]	余丽莹,黄宝优,谭小明,等.广西两面针野生种质资源调查研究[J].广西植物,2009,29(2):231-235.
[3]	葛槐发,葛爱发.两面针在中医急症中的临床应用[J].时珍国药研究,1995,6(4):6-7.
[4]	黄光伟. 含两面针的漱口液[J].牙膏工业,2002(2):32-34.
[5]	赖茂祥,饶伟源,严克俭,等.两面针茎及其伪品的生药鉴别[J].中药材,1994,17(3):18-21.
[6]	刘绍华,覃青云,唐献兰,等.两面针的药学研究与开发利用[J].广西科学院学报,2005,21(2):130-132.
[7]	汤丽云,何蔚思,何国振.两面针的愈伤组织诱导与分化[J].天然产物研究与开发,2017,29(8):1290-1295.
[8]	韦大器,吴红英,何贵整,等.两面针的组织培养和快速繁殖[J].植物生理学通讯,2006,42(1):73.
[9]	莫勇生,孙文波,张红岩.两面针的组织培养研究[J].农业研究与应用,2012(6):16-20.
[10]	蒋益虹. 百合褐变与多酚氧化酶和过氧化物酶活性关系的研究[J].浙江大学学报,2003,29(5):518-522.
[11]	周俊辉,周家容,曾浩森,等.园艺植物组织培养中的褐化现象及抗褐化研究进展[J].园艺学报,2000,27(S):481-486.
[12]	田如英,刘燕,祁翔,等.兔眼蓝莓外植体抗褐化技术初探[J].北方园艺,2009(12):88-90.
[13]	张爱萍. 活性炭在植物组织培养中的应用[J].中国果菜, 2009(2):42.
[14]	梅兴国,董妍玲,潘学武.红豆杉细胞继代培养防褐变措施的研究[J].天然产物研究与开发,2001,13(4):8-11.
[15]	刘均利,马明东.华盖木组织培养中褐化控制研究[J].浙江林业科技,2007,27(1):20-23.