Abstract: With the bulbs of Lillium spp. var. Khaki as explants, MS medium plus different concentrations of plant growth regulators were used to inducing adventitious shoots and roots for plant regeneration. In this process, orthogonal experimental methods were used to analysis the impact of the concentrations and proportions of 6-BA, NAA, GA, IBA and active carbon to shoots and roots inducement of Lillium spp. var. Khaki. The results showed that the optimal medium for shoots inducement was MS+2.0 mg/L 6-BA+0.1 mg/L NAA+0.5 mg/L GA₃. After retesting, it was proved that the ratio of shoots inducement was 83.3% and the shoots were strong, fresh green and less browning. The optimal medium for rooting of adventitious shoots was 1/2MS+0.1 mg/L NAA+0.1 mg/L IBA and the ratio of rooting was 73.3%. The number of roots for each adventitious shoot was higher. The roots were strong and in good condition.

Key words: Lillium spp. var. Khaki, tissue culture, plant growth regulator, adventitious shoot