Genetic Diversity Analysis of Iris L. Germplasm by SRAP

doi:10.14088/j.cnki.issn0439-8114.2019.04.020

Abstract

Abstract: Sequence-related amplified polymorphism （SRAP） was used to detect the genetic diversity among 40 Iris L. materials. 10 primers which could produce clear, polymorphic and steady bands, screened out of 170 random primers, which were used and produced 168 bands totally. All of bands were polymorphic（100%）, which indicated that the genetic diversity of Iris L. was rich. The genetic similarity coefficient among the accessions ranged from 0.067 to 0.740, and the average was 0.281. At the point of the similarity coefficient 0.265, all the accessions might be separated into seven groups. 19 accessions of Iris germanica were brought together. Iris confusa Sealy, Iris halophila and Iris germanica ‘Queen’ were classified separately. Iris lactea Pall. var. chinensis from Shanghai and Ningxia were lumped into the same category, while Iris lactea Pall. var. chinensis from Inner Mongolian and Iris louisiana were lumped together. Iris sibirica, Iris ensata, Iris pseudacorus and three accessions of Iris germanica were lumped into the same category. The results of principal coordinates showed similarity to the UPGMA dendrogram.

Key words: Iris L., SRAP, genetic diversity, relationship

CLC Number:

S682.1⁺9

TONG Jun, MAO Jing, DONG Yan-fang, XU Dong-yun, FANG Lin-chuan, GUO Cai-xia, WANG Yan, ZHOU Yuan. Genetic Diversity Analysis of Iris L. Germplasm by SRAP[J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(4): 88-92.

References 12

[1]	赵毓棠. 中国植物志[M].北京:科学出版社,1985.
[2]	赵毓棠. 鸢尾欣赏与栽培利用[M].北京:金盾出版社,2005.
[3]	胡永红,肖月娥.湿生鸢尾——品种赏析、栽培及应用[M].北京:科学出版社,2012.
[4]	牟少华,郄光发,彭镇华,等.我国鸢尾属植物种质资源的研究与利用[J].草业科学,2007,24(8):21-24.
[5]	张敏,黄苏珍.鸢尾属部分植物种质资源的ISSR分析[J].南京农业大学学报,2008,34(5):58-62.
[6]	廖芳蕾,陈民管,桑丹,等.佛手种质资源遗传多样性的ISSR分析[J].园艺学报,2013,40(11):2222-2228.
[7]	叶要妹. 利用RAPD和ISSR分析百日草自交系间的种质遗传多样性[J].中南林业科技大学学报,2008,28(4):36-41.
[8]	宋明,刘婷,汤青林,等.芥菜种质资源的RAPD和ISSR分析[J].园艺学报,2009,36(6):835-842.
[9]	孙明伟,袁素霞,徐有明,等.基于SRAP标记的百合部分种及品种遗传结构分析[J].园艺学报,2011,38(8):1498-1506.
[10]	王瑞芬. 利用SSR和SRAP分子标记对新疆喜盐鸢尾居群遗传多样性的研究[D].乌鲁木齐:新疆农业大学,2010.
[11]	李承哲,刘占林,杨红超,等.改良CTAB法提取鸢尾属植物DNA[J].现代农业科技,2011(8):31,34.
[12]	童俊,周媛,毛静,等.鸢尾属部分园艺品种遗传多样性的ISSR分析[J].北方园艺,2015(10):104-107.

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