Bacteriostatic effect in vitro of lactic acid treated water and its influence on serum indexes and fecal pH of Sanhuang chicken

doi:10.14088/j.cnki.issn0439-8114.2022.19.021

Abstract

Abstract: In order to study the bacteriostatic effect of lactic acid treated water in vitro and its effect on the serum index and fecal pH of Sanhuang chicken, the plate counting method was used to observe the inhibitory effect of lactic acid with different pH on Escherichia coli and Salmonella in water. The test was divided into lactic acid treated sterile water group, sterile water group, antibiotic group and blank control group. After culturing for 2, 8, 14, 20 and 24 hours, the number of colonies was counted respectively. According to the in vitro bacteriostatic results, 120 1-day-old Sanhuang chickens were selected and fed in the control group and the lactate drinking water group. Serum total protein （TP）, albumin （ALB）,globulin（GLB） and γ-glutamyl transpeptidase （GGT） were measured and the fecal pH was recorded every day. The results showed that, ① when the pH of lactic acid treated water was 1~2, 2~3 and 3~4, the colony number of Escherichia coli and Salmonella was 0 at the beginning of 2 hours of culture. When the pH was 4~5, the number of colonies on the plate of the two strains decreased significantly after 8 hours of culture. When the pH was 5~6, the colony numbers of Escherichia coli and Salmonella decreased significantly at 24 h and 14 h respectively. ② TP and GLB of 13-day-old and 25-day-old Sanhuang chickens in the lactate drinking group were significantly higher than those in the control group （P<0.05）; ALB was higher than that in the control group （P>0.05）; GGT and A/G were not significantly different （P>0.05）. Compared with the control group, there was no significant difference in fecal pH （P> 0.05）, but there was a downward trend. It could be seen that when the pH of the lactic acid treated water was 1~4, it could effectively inhibit Escherichia coli and Salmonella in vitro. Drinking the lactic acid treated water with pH 3~4 was beneficial to the production of TP,ALB and GLB,which could promote the health of chickens, enhance the immunity of the body, and adjust the pH of the digestive tract.

Key words: lactic acid, antibacterial effect in vitro, Sanhuang chicken, serum index, fecal pH

CLC Number:

S831.4

WANG Shu-yan, SHEN Qian-cheng, QIN Jun, LI Xiao-yan, SHU Yong-chun, LING Ding. Bacteriostatic effect in vitro of lactic acid treated water and its influence on serum indexes and fecal pH of Sanhuang chicken[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(19): 110-113.

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