EMS mutagenesis technology of watermelon seeds

doi:10.14088/j.cnki.issn0439-8114.2022.24.021

Abstract

Abstract: Three mutagenesis times （4, 8, 12 h） and six mutagenesis concentrations （0%, 1.5%, 2.0%, 3.0%, 4.0%, 5.0%） were set to treat high generation self-pollination watermelon ‘97103' seeds with ethyl methyl sulfonate （EMS） as mutagenesis agent. The suitable combinations were selected according to the germination rate and seedling rate of watermelon treated with mutagenesis. The results showed that the germination rate and seedling rate of ‘97103' showed a decreasing trend with the increase of EMS mutagenic concentration and the increase of treatment time. To ensure a certain number of surviving plants and an adequate mutant community, a combination of conditions with germination and seedling rate close to half lethal dose （5.0% EMS solution treatment for 8 h） was used as a more ideal treatment condition for ‘97103' mutagenesis.

Key words: watermelon seed, EMS mutagenesis, mutant

CLC Number:

S651

SHEN Sai-ya, ZENG Hong-xia, REN Jian, CHAI Shan-shan, SUN Yu-hong, ZHANG Na. EMS mutagenesis technology of watermelon seeds[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(24): 95-99.

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