Establishment of a rapid propagation system for callus regeneration and seedling formation of Uncaria rhynchophylla

doi:10.14088/j.cnki.issn0439-8114.2025.12.025

Abstract

Abstract: Using the tender stem segments of Uncaria rhynchophylla as experimental materials, tissue culture techniques including explant surface sterilization, browning control, callus induction and differentiation, and adventitious root induction were systematically studied. The results showed that the optimal sterilization method for Uncaria rhynchophylla explants was soaking in 75% ethanol for 30 seconds, rinsing with sterile water 3 times, followed by treatment with 0.1% HgCl₂ solution for 8 minutes. The addition of 7 mg/L ascorbic acid (VC) effectively reduced explant browning. The optimal medium for callus induction was MS+1.0 mg/L 6-BA+1.5 mg/L 2,4-D; the optimal differentiation medium was MS+0.2 mg/L NAA+2.5 mg/L 6-BA; and the optimal medium for adventitious root induction was 1/2 MS+1.5 mg/L NAA.

Key words: Uncaria rhynchophylla, callus induction, browning, rapid propagation system

CLC Number:

PAN Li-mei, HUANG Yuan, ZHAN Xin-jie, WAN Ling-yun, WEI Shu-gen, JIANG Ni, XIAO Xiao, LIU Huan-ying, QIAO Le-wen, LI Lin-xuan, ZHANG Zhan-jiang. Establishment of a rapid propagation system for callus regeneration and seedling formation of Uncaria rhynchophylla[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(12): 152-155.

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