Study on super high pressure assisted deep eutectic solvent extraction of total flavonoids from Rabdosia rubescens (Hemsl.) Hara and its antioxidant activity

doi:10.14088/j.cnki.issn0439-8114.2025.12.031

Abstract

Abstract: To optimize the extraction process of total flavonoids from Rabdosia rubescens (Hemsl.) Hara in super high pressure assisted deep eutectic solvent (DES) and analyze its antioxidant activity, the extraction condition was optimized by single factor experiments and response surface experiments. The scavenging activity of the extracted flavonoids against 1,1-dipyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-ulfonic acid) diammonium salt (ABTS) free radicals was analyzed. The results showed that the optimal condition for the extraction of total flavonoids in super high pressure assisted deep eutectic solvent was material to liquid ratio of 1∶; 40 (g/mL), water content in DES of 30% ,extraction pressure of 402 MPa, and extraction time of 5 min, under which the yield of total flavonoids from the Rabdosia rubescens (Hemsl.) Hara was 9.21%. The half-inhibitory concentrations of the prepared total flavonoids of Rabdosia rubescens (Hemsl.) Hara for the scavenging effects of DPPH· and ABTS·were 0.136 mg/mL and 0.123 mg/mL, respectively. The research could provide theoretical support for the development of total flavonoids of Rabdosia rubescens (Hemsl.) Hara and the study of antioxidant activity.;

Key words: Rabdosia rubescens (Hemsl.) Hara, total flavonoids, super high pressure, deep eutectic solvent, antioxidant

CLC Number:

R284.2

WANG Le, ZHANG Pei-qi, ZONG Wei. Study on super high pressure assisted deep eutectic solvent extraction of total flavonoids from Rabdosia rubescens (Hemsl.) Hara and its antioxidant activity[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(12): 181-186.

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