Purification process and in vitro antioxidant activity of procyanidins from Embelia parviflora Wall.

doi:10.14088/j.cnki.issn0439-8114.2025.06.026

Abstract

Abstract: The purification process of proanthocyanidins from Embelia parviflora Wall. was studied by using polyamide resin chromatography, and the static and dynamic adsorption and desorption capacities of polyamide resin were investigated. The antioxidant capacity of the purified proanthocyanidins from Embelia parviflora was also evaluated. The results showed that the optimized purification process was as follows: The sample loading concentration was 1.88~3.21 mg/mL, the sample loading flow rate was 2~3 mL/min, the dynamic specific adsorption capacity of polyamide was 16.05 mg/g, and the optimal elution flow rate was 2~3 mL/min. The elution rates of different volume fractions of desorption solutions were: 50% ethanol solution > 70% ethanol solution > 30% ethanol solution > 95% ethanol solution. In the antioxidant test, the scavenging rates of ABTS⁺· and the total reducing power of proanthocyanidins from Embelia parviflora and the proanthocyanidin reference substance were close to those of vitamin C, but the scavenging rates of DPPH· and superoxide anion were lower than those of vitamin C.

Key words: Embelia parviflora Wall, proanthocyanidins, polyamide resin, purification process, antioxidant activity

CLC Number:

R962

FAN Li-hua, LI Jin-zhou, LI Meng-qi, ZHOU Guang-yun, DENG Qing-mei, WEI Jiang-cun, CHEN Yong. Purification process and in vitro antioxidant activity of procyanidins from Embelia parviflora Wall.[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(6): 155-160.

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