Genetic mapping analysis of interspecific hybrid population in buckwheat

doi:10.14088/j.cnki.issn0439-8114.2025.05.026

HUBEI AGRICULTURAL SCIENCES ›› 2025, Vol. 64 ›› Issue (5): 167-172.doi: 10.14088/j.cnki.issn0439-8114.2025.05.026

• Biological Engineering • Previous Articles Next Articles

Genetic mapping analysis of interspecific hybrid population in buckwheat

YIN Gui-fang¹, LU Wen-jie¹, LONG Wen-jie¹, SUN Dao-wang¹, HUANG Chun-yan², ZHAI Bing-xin², WANG Li-hua¹

1. Biotechnology and Genetic Germplasm Resources Research Institute/Yunnan Provincial Key Laboratory of Agricultural Biotechnology/Key Laboratory of Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, Yunnan Academy of Agricultural Sciences, Kunming 650205, China;
2. Institute of Resource Plants, Yunnan University, Kunming 650500, China

Received:2024-05-09 Online:2025-05-25 Published:2025-06-11

Abstract

Abstract: An F₂ population was constructed by crossing the cultivated buckwheat（Fagopyrum esculentum Moench.） variety Yunqiao 1 (YQ1) with the lodging-resistant and disease-resistant wild buckwheat YZ56,extreme phenotypic individuals for traits including plant height, grain weight per plant, and leaf blight resistance were screened based on agronomic trait investigation data,and DNA was extracted from buckwheat leaves using the CTAB method to construct extreme phenotypic DNA pools. The results demonstrated that plant height and grain weight per plant in the F₂ population showed normal distributions,with individuals exhibiting lower leaf blight incidence occupying a higher proportion in the distribution. The average grain weight per plant of the F2 population was 17.8 g, higher than that of the parental lines YZ56 (13.1 g) and YQ1 (16.7 g),indicating significant heterosis. Rational utilization of distant germplasm resources for hybridization could improve progeny yield.Pearson correlation analysis revealed highly significant correlations among plant height, grain weight per plant, and leaf blight incidence rate. Pooled sequencing combined with bulked segregant analysis (BSA) successfully identified 10 QTL regions associated with plant height at a 95% confidence interval,7 candidate QTL regions related to grain weight per plant,and 6 candidate QTL regions linked to leaf blight resistance.

Key words: buckwheat（Fagopyrum esculentum Moench.）, interspecific hybrid population, genome sequencing, gene mapping

CLC Number:

S334

YIN Gui-fang, LU Wen-jie, LONG Wen-jie, SUN Dao-wang, HUANG Chun-yan, ZHAI Bing-xin, WANG Li-hua. Genetic mapping analysis of interspecific hybrid population in buckwheat[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(5): 167-172.

References

[1] 唐宇,邵继荣,周美亮.中国荞麦属植物分类学的修订[J].植物遗传资源学报,2019,20(3):646-653.
[2] 范昱,丁梦琦,张凯旋,等.荞麦种质资源概况[J].植物遗传资源学报,2019,20(4):813-828.
[3] 范昱,丁梦琦,张凯旋,等.中国野生荞麦种质资源概况与利用进展[J].植物遗传资源学报,2020,21(6):1395-1406.
[4] 内蒙古农业科学院,中国农业科学院作物品种资源研究所.中国荞麦品种资源目录(第一辑)[M].北京:中国农业出版社,1986.
[5] 中国农业科学院作物品种资源研究所.中国荞麦品种资源目录(第二辑)[M].北京:中国农业出版社,1996.
[6] 赵建栋,李秀莲,陈稳良,等. 我国荞麦育种成就、问题及对策[J].种子,2017,36(4):67-71.
[7] 王莉花,王艳青,卢文洁,等.云荞1号的选育及高产栽培技术[J].山西农业科学,2012,40(8):829-832.
[8] TAKESHIMA R, OGISO-TANAKA E, YASUI Y, et al.Targeted amplicon sequencing + next-generation sequencing-based bulked segregant analysis identified genetic loci associated with preharvest sprouting tolerance in common buckwheat (Fagopyrum esculentum)[J].BMC plant biology,2021, 21(1):18.
[9] DUAN Y, YIN G, HE R,et al. Identification of candidate genes for easily-shelled traits in Tartary buckwheat based on BSA-Seq and RNA-Seq methods[J].Euphytica, 2022,218, 91:42.
[10] 汪灿,阮仁武,袁晓辉,等.荞麦茎秆解剖结构和木质素代谢及其与抗倒性的关系[J].作物学报,2014,40(10):1846-1856.
[11] 汪灿,阮仁武,袁晓辉,等.不同荞麦品种抗倒伏能力与根系及茎秆性状的关系[J].西南大学学报(自然科学版),2015,37(1): 65-71.
[12] 李英双,胡丹,聂蛟,等.甜荞株高和茎粗的遗传分析[J].作物学报,2018,44(8):1185-1195.
[13] 胡丹. 甜荞茎秆重心高度和抗折力的遗传分析[J].作物杂志, 2022 (4): 83-89.
[14] SHEN Q, PENG X X, HE F,et al.First report of Nigrospora osmanthi causing leaf spot on tartary buckwheat in China[J].Plant disease, 2021, 105: 1227.
[15] 卢文洁,罗志明,李春花,等.云南省荞麦叶枯病病原菌鉴定及其生物学特性[J].植物保护学报,2019,46(5):1065-1072.
[16] 李月,石桃雄,黄凯丰,等.苦荞生态因子及农艺性状与产量的相关分析[J].西南农业学报,2013,26(1):35-41.
[17] 陈庆富,陈其饺,石桃雄,等. 苦荞厚果壳性状的遗传及其与产量因素的相关性研究[J].作物杂志,2015 (2):27-31.
[18] 陈稳良, 李秀莲, 史兴海, 等.甜荞产量性状综合评价及AMMI分析[J].核农学报, 2023, 37(1): 60-68
[19] ZHANG L, LI X, MA B, et al.The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance[J].Molecular plant,2017, 10(9):1224-1237.
[20] HE Q, MA D, LI W, et al.High-quality Fagopyrum esculentum genome provides insights into the flavonoid accumulation among different tissues and self-incompatibility[J].Journal of integrative plant biology,2023,6: 1423-1441.

Genetic mapping analysis of interspecific hybrid population in buckwheat

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments

[1]	YIN Chun, GUO Lai-chun, WU Jun-ying, LIU Jin-quan, LU Fu-kuan, LIU Jing-hui. Identification and evaluation of agronomic traits of sweet buckwheat germplasm resources in the Hohhot-Baotou area of Inner Mongolia Autonomous Region [J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(9): 1-5.
[2]	ZHANG Wan-yu, LIU Bao-guo, WEI Hong. Analysis and map construction of traditional villages from the perspective of landscape gene： Taking Qianxiewan village in Pingdingshan city as an example [J]. HUBEI AGRICULTURAL SCIENCES, 2021, 60(11): 93-98.
[3]	ZHAO Xia-xia, WANG Xu-ming, XU Piao, ZHAO Li-na, HU Yan, CHEN Jing-yang, HUANG Yong-xiang, LI Wei, GUO Jiang-fu. Research and prospect of rice blast resistance [J]. HUBEI AGRICULTURAL SCIENCES, 2019, 58(11): 5-9.
[4]	MU Yang-xiu, YANG Li-juan, ZHANG Jiu-pan, MU Lan-hai, DU Yan-ping, CHANG Ke-qin. Effect of Planting Density on Buckwheat Seed Setting Rate and Yield [J]. HUBEI AGRICULTURAL SCIENCES, 2018, 57(2): 32-34.