Identification and expression pattern analysis of the calmodulin-like protein gene family in rice

doi:10.14088/j.cnki.issn0439-8114.2026.05.033

Abstract

Abstract: To systematically identify the members of the rice calmodulin-like protein (OsCML) gene family and explore their potential functions in growth, development, and abiotic stress responses, bioinformatics methods were used to identify a total of 44 OsCML genes at the whole-genome level, and their physicochemical properties, phylogeny, gene structures, duplication events, syntenic relationships, cis-acting elements, and expression patterns were comprehensively analyzed. The results showed that the 44 OsCML genes were unevenly distributed on 12 chromosomes, and most OsCML proteins were localized in the cytoplasm and cell membrane.Phylogenetic analysis divided the OsCML proteins into eight subfamilies. Gene duplication analysis showed that segmental duplication was the main driving force for the expansion of this family. Interspecific synteny analysis revealed that CML genes had highly conserved syntenic relationships between monocot and dicot plants. Cis-acting element analysis indicated that the promoter regions of OsCML genes were mainly enriched with four types of elements related to light response, stress response, hormone response, and growth and development. The OsCML gene family showed specific and widespread expression differences in seven tissues, and exhibited diverse responses under four abiotic stresses, suggesting that they played key roles in the stress adaptation of rice.

Key words: rice, calmodulin-like protein, gene family, identification, expression pattern

CLC Number:

S511

YANG Lan-tian, YU Zhen-yuan, XIE Yi. Identification and expression pattern analysis of the calmodulin-like protein gene family in rice[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(5): 221-229.

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