Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis

doi:10.14088/j.cnki.issn0439-8114.2020.22.015

Abstract

Abstract: To screen bacteria capable of decolorizing malachite green (MG) from the soil-derived bacteria,as a result,a strain of Enterobacter cloacae (S1-2) had a strong ability to decolorize MG.According to single variable principle,the different conditions that affected the strain S1-2 were characterized by the change in absorbance of the solution before and after MG degradation.The results showed that the strain S1-2 could completely decolorize1100 mg/L MG within36 h,and the inhibitory rate of treated MG on Bacillus amyloliquefaciens and Bacillus parvii decreased by43.98%~48.21%.The analysis of decolorization conditions with300 mg/L MG found that the degradation rate of 0.5%~7.0% inoculation amount of strain S1-2 was91.67%~93.91%;In different pH culture environment,the strain S1-2 decolorized90.02%~94.54% MG at pH6 and pH7;The strain S1-2 decomposed84.61%~94.56% MG,when cultured at20~40 ℃;Under the concentration of6~18 g/L NaCl,the strain S1-2 degraded91.85%~93.77% MG.

Key words: malachite green, Enterobacter cloacae, toxicity test, decolorization characteristics

CLC Number:

X172
X788

LIAO Hua-yuan, XIONG Meng-xia, LI Run-cheng. Screening of soil-derived bacteria decolorizing malachite green and decoloring condition analysis[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(22): 86-90.

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