A rapid and sensitive method for evaluating aging life of rice seeds

doi:10.14088/j.cnki.issn0439-8114.2022.19.001

Abstract

Abstract: The seeds of 9 rice varieties （Saika, IR30, Bra80sci9875, Koshihikari, Gangyuan, Nipponbare, Jinjing 818, Jindao 919, Jindao 372） were used as experimental materials. The seeds were treated with water bath at different temperatures and different times. The germination test was carried out after soaking in the artificial climate culture room for 24 h. The germination rate, root length, bud length and vigor were measured. The results showed that when the treatment temperature was 50 ℃, the average germination rate of five rice varieties （Saika, Koshihikari, Gangyuan, Nipponbare, Jindao 372） was significantly different, and the proportion of Nipponbare varieties reaching G₅₀ was 60.00%, which could accurately distinguish the germination rate of different rice varieties. With the extension of water bath time, the average germination rate of rice varieties showed a downward trend. The aging rate of Jindao 372 was the fastest, and the average germination rate was almost 0 after 4 h treatment. When treated for 4 h, more than 50.00% of the rice varieties reached G₅₀, and the average germination rate distribution of the nine rice varieties was relatively uniform, with the order of Saika>Koshihikari>Nipponbare>Jindao 919>IR30>Gangyuan>Bra80sci 9875>Jinjing 818>Jindao 372. Accelerated aging testing at 50 ℃ for 4 h in a water bath is a fast and sensitive method that can be completed within 7 days.

Key words: temperature, time, germination, seed vigor

CLC Number:

GUO Yan-li, TONG Hui, LIU Yan-qing, LU Xin, ZHANG Hong-lei, YAN Shuang-yong, ZHANG Rong-xue, SU Jing-ping, LIU Xue-jun. A rapid and sensitive method for evaluating aging life of rice seeds[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(19): 5-10.

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