The physiological response of Paulownia fortunei 1201 seedlings under plumbum and cadmium stress

doi:10.14088/j.cnki.issn0439-8114.2022.20.016

Abstract

Abstract: A series of changes of seedling biomass, physiological and biochemical indexes and subcellular distribution of Paulownia fortunei 1201 under plumbum and cadmium stress were studied by hydroponic experiment. The results showed that the effect of plumbum and cadmium stress on seedling biomass of Paulownia fortunei 1201 was promoting at low level and suppressing at high level. Paulownia fortunei 1201 seedlings had stronger absorption and enrichment of cadmium than plumbum, and the heavy metal content in each part of the seedlings was in the order of root > leaf > stem. The bioenrichment coefficient and transport coefficient in each part of the seedlings showed a downward trend. The root of Paulownia fortunei 1201 seedlings played a role of buffer and protective barrier, becoming the main part of absorption and enrichment of plumbum and cadmium. Under plumbum and cadmium stress, super oxide dismutase（SOD） activities of Paulownia fortunei 1201 seedling roots decreased first and then increased with the increasing of stress concentration, while SOD activities of Paulownia fortunei 1201 seedling leaves had no significant change. Leaf catalase（CAT） activities increased first and then decreased with the increase of stress concentration. Peroxidase（POD） activities were positively correlated with stress concentration. Soluble sugar contents increased firstly and then decreased with the increasce of stress concentration, and the soluble protein contents increased first and then decreased, and free proline contents were positively correlated with stress concentration. With the increase of stress concentration, non-protein thiols compounds（NPT） contents increased first and then decreased, glutathione（GSH） contents decreased, phytochelatins（PCs） contents showed more complicated changes, but generally increased first and then decreased, and the contents of non-protein thiols compounds in leaves were significantly higher than those in roots. The accumulation of Cd²⁺ and Pb²⁺in subcellular distribution was positively correlated with stress concentration.

Key words: Paulownia fortunei 1201, biomass, antioxidant enzyme, non-protein thiols compounds, subcellular distribution

CLC Number:

ZHU Xiu-hong, ZHANG Ji-zhong, LI Zhe-jing, ZHANG Shu-jing, RU Guang-xin. The physiological response of Paulownia fortunei 1201 seedlings under plumbum and cadmium stress[J]. HUBEI AGRICULTURAL SCIENCES, 2022, 61(20): 82-91.

References

[1] 李燕,马瑜,朱海云,等.重金属污染土壤植物及其联合修复的研究进展[J].环境科学与技术,2016,39(S2):299-303.
[2] 茹广欣. 泡桐遗传变异与改良研究[D].北京:中国林业科学研究院,2004.
[3] 毛碧辉,吕成群,黄宝灵,等.不同造林密度对泡桐幼林生长和林分蓄积量的影响[J].安徽农业科学,2018,46(20):102-105.
[4] 王利美,邓敏捷,张艳芳,等.泡桐丛枝病相关microRNAs测序及其靶基因预测[J].森林与环境学报,2021,41(2):180-187.
[5] 吕亭亭,杨志华,陶娟,等.泡桐花总黄酮的提取工艺优化及抗氧化活性[J].中国酿造,2021,40(1):197-202.
[6] 朱秀红,赵珠琳,程红梅,等.不同品种泡桐制备乙醇的预处理工艺优化研究[J].林产工业,2021,58(3):52-56.
[7] 刘畅,徐应明,黄青青,等.不同冬小麦品种镉富集转运及离子组特征差异[J].环境科学,2022,43(3):1596-1605.
[8] XU Q Z,HUANG B R.Antioxidant metabolism associated with summer leaf senescenceand turf quality decline for creeping bentgrass[J].Crop science,2004,44(2):553-560.
[9] 李合生. 植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[10] 张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.
[11] 赵亚华. 生物化学实验技术教程[M].广州:华南理工大学出版社,2000.
[12] 王学奎. 植物生理生化实验原理和技术[M].北京:高等教育出版社,2006.176-286.
[13] WANG H,GONG M,XIN H,et al.Effects of chilling stress on the accumulation of soluble sugars and their key enzymes in Jatropha curcas seedlings[J].Physiology and molecular biology of plants,2018,24(5):857-865.
[14] KELTJENS W G,VAN B M L. Phytochelatins as biomarkers for heavy metal stress in maize(Zea mays L.)and wheat(Triticum aestivum L.): Combined effects of copper and cadmium[J].Plant and soil,1998,203(1):119-126.
[15] XIN W,LIU Y G,ZENG G M,et al.Subcellular distribution and chemical forms of cadmium in Bechmeria nivea(L.)Gaud[J].Environmental and experimental botany,2008,62(3):389-395.
[16] IBRAHIM E A.Seed priming to alleviate salinity stress in germinating seeds[J].J Plant Physiol,2016,192:38-46.
[17] 宋香静,李胜男,郭嘉,等.不同盐分水平对柽柳扦插苗根系生长及生理特性的影响[J].生态学报,2018,38(2):606-614.
[18] 刘凤,魏雨蒙,刘霜平,等.镉胁迫对小麦生长和生理特性的影响[J].山东化工,2017,46(3):24-26.
[19] 惠俊爱,党志.土壤不同镉浓度对玉米CT38生长及抗氧化酶活性的影响[J].生态环境学报,2014,23(5): 884-889.
[20] PATRA M,BHOWMIK N,BANDOPADHYAY B,et al.Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance[J].Environmental and experimental botany,2004,52(3):199-223.
[21] 周振,杨素勤,柳海涛,等.曼陀罗对镉的吸收及其亚细胞分布研究[J].农业资源与环境学报,2019,36(3):385-391.
[22] 檀建新,尹君,王文忠,等.镉对小麦、玉米幼苗生长和生理生化反应的影响[J].河北农业大学学报,1994(S1):83-87.
[23] 阎雨平,蔡士悦,史艇.广东赤红壤、红壤含镉的农作物污染效应及其临界含量研究[J].环境科学研究,1992(2):49-53.
[24] 石元值,阮建云,马立峰,等.茶树中镉、砷元素的吸收累积特性[J].生态与农村环境学报,2006(3):70-75.
[25] 毛永成,刘璐,王小德.干旱胁迫对3种槭树科植物生理特性的影响[J].浙江农林大学学报,2016,33(1): 60-64.
[26] 谢亚兵,林铃,毕学琴,等. Cd、Pb 单一胁迫对芳樟小苗叶片生理指标的影响[J].安徽农学通报,2018, 24(6):94-96,103.
[27] 郭晖,郭孝茹,柴光东,等.重金属短期胁迫下5种观赏植物积累特性与生理抗性研究[J].西南林业大学学报(自然科学),2017,37(4):28-34.
[28] JIANG J L,SU M,CHEN Y R,et al.Correlation of drought resistance in grass pea (Lathyrus sativus) with reactive oxygen species scavenging and osmotic adjustment[J].Biologia,2013,68(2):231-240.
[29] 汪本福,王晴芳,李阳,等.干旱胁迫对水稻叶片生理生化特性的影响综述[J].湖北农业科学,2019,58(23):5-9,30.
[30] ACA O,TURKAN I, ÖZDEMIR F.Superoxide dismutase and peroxidase activities in drought sensitive andresistant barley (Hordeum vulgare L.) varieties[J].Acta physiologiae plantarum,2001, 23(3):351-356.
[31] ATTA K,PAL A K,JANA K.Effects of salinity, drought and heavy metal stress during seed germination stage in ricebean [Vigna umbellata (Thunb.) Ohwi and Ohashi][J].Plant physiology reports,2020,7:1-7.
[32] 焦德志,赵泽龙.盐碱胁迫对植物形态和生理生化影响及植物响应的研究进展[J].江苏农业科学,2019,47(20):1-4.
[33] 刘金萍,高奔,李欣,等.盐旱互作对不同生境盐地碱蓬种子萌发和幼苗生长的影响[J].生态学报,2010,30(20):5485-5490.
[34] 查应琴,潘凤,关萍.镉胁迫对鸡冠花种子萌发及幼苗生理生化特性的影响[J].西北植物学报,2020,40(11):1900-1908.
[35] 张思悦,张晴,李凌.黄葛树对土壤铅、镉污染耐受性的研究[J].西南师范大学学报(自然科学版),2019,44(1):79-83.
[36] DAI H P,WEI S H,TWARDOWSKA I,et al.Hyperaccumulating potential of Bidens pilosa L. for Cd and elucidation of its translocation behavior based on cell membrane permeability[J].Environmental science and pollution research,2017,24(29):23161-23167.
[37] YANG H C,CUI J,LUO C F,et al.Progress in metabolism of proline and sugar beet stress tolerance[J].China beet sugar,2015(4):30-35.
[38] 张方静,罗峰,谭殷殷,等.高温胁迫对月季生理特性和叶绿素荧光参数的影响[J].河南农业科学,2019,48(4):108-115.
[39] WENG B,XIE X Y,WEISS D J,et al.Kandelia obovata (S., L.) Yong tolerance mechanisms to cadmium:Subcellular distribution, chemical forms and thiol pools[J].Marine pollution bulletin,2012,64(11):2453-2460.
[40] WEI Z G,WONG J W,CHEN D.Speciation of heavy metal binding non-protein thiols in Agropyron elongatum by size-exclusion HPLC-ICP-MS[J].Microchemical journal,2003,74(3):207-213.
[41] SUN Q,WANG X R,DING S M,et al.Effects of interactions between cadmium and zinc on phytochelatin and glutathione production in wheat (Triticum aestivum L.)[J].Environmental toxicology: An international journal, 2005,20(2):195-201.
[42] VAZQUEZ S,GOLDSBROUGH P,CARPENA R O.Comparative analysis of the contribution of phytochelatins to cadmium and arsenic tolerance in soybean and white lupin[J].Plant physiology and biochemistry,2008, 47(1):63-67.
[43] MAHDAVIAN K,GHADERIAN S M,SCHAT H.Pb accumulation, Pb tolerance, antioxidants, thiols, and organic acids in metallicolous and non-metallicolous Peganum harmala L. under Pb exposure[J].Environmental and experimental botany,2016,126:21-31.
[44] BHOOMIKA K,PYNGROPE S,DUBEY R S.Effect of aluminum on protein oxidation, non-protein thiols and protease activity in seedlings of rice cultivars differing in aluminum tolerance[J].Journal of plant physiology,2014, 171(7):497-508.
[45] LAI H Y.Subcellular distribution and chemical forms of cadmium in Impatiens walleriana in relation to its phytoextraction potential[J].Chemosphere,2015,138:370-376.
[46] 李杉杉. 镉污染土壤高效钝化-植物阻控效果与机理研究[D].北京:中国地质大学(北京),2019.
[47] 史静,潘根兴.外加镉对水稻镉吸收、亚细胞分布及非蛋白巯基含量的影响[J].生态环境学报,2015,24(5):853-859.
[48] 张海利,王涛,邹路易,等.铜绿假单胞菌对龙葵中Cd的亚细胞分布和化学形态的影响[J].农业环境科学学报,2018,37(8):1602-1609.