Abstract: The Ampelopsis grossedentata （AG） composite juice was prepared, followed by investigating its anti-thrombotic effect in rats. The AG composite juice was used to constantly intervene in the thrombus rats model, that was successfully established by using ligation of the inferior vena cava. The DHM solution and AG juice were used as controls. Rats were then sacrificed prior to determining the weight, length of the thrombus samples, and the antithrombotic rate. Consequently, the physicochemical properties of thrombus samples were measured by using HE staining and immunohistochemistry. Results showed that the content of total flavonoids （1.43±0.11 mg/mL） and DHM （0.66±0.05 mg/mL） in composite juice were significantly lower than those in control groups. Moreover, the length （10.98±1.20 mm）, wet weight （70.6±15.4 mg） and dry weight （49.9±3.2 mg） of thrombus in the model group were obviously higher than those in the normal group. Compared with other invention groups, the length of thrombus （3.92±0.64 mm） and the ratio of thrombus area to the entire inner surface area of blood vessels （32.1±1.4）% decreased significantly in the AG composite juice group, while the thrombus inhibition rate （64.5±1.3）% and dissolution rate （54.2±1.3）% increased significantly. Additionally, the HE staining and immunohistochemistry indicated that the AG composite juice group exhibited lower degree of vascular cell damage in integrity and nuclear fixation, relative expression level （25.2±2.1）% of TBXAS1, as well as a higher positive cell rate compared to other intervention groups. The AG composite juice had a favorable anti-thrombotic effect.

Key words: Ampelopsis grossedentata, dihydromyricetin, thrombosis, thromboxane