HUBEI AGRICULTURAL SCIENCES ›› 2020, Vol. 59 ›› Issue (4): 98-101.doi: 10.14088/j.cnki.issn0439-8114.2020.04.020

• Horticulture & Local Products • Previous Articles     Next Articles

Study on flavonoids from Ampelopsis grossedentata in Lichuan city

LI Yu1, LIU Cui-jun1, AI Lun-qiang1, ZHANG Yu1, LU Xin-peng2, HE Mei-jun1,2, RAN Ya-lan3   

  1. 1.Institute of Chinese Herbal Medicines,Hubei Academy of Agricultural Sciences,Enshi 445000,Hubei,China;
    2.Medical School,Hubei Minzu University,Enshi 445000,Hubei,China;
    3.Agricultural and Rural Bureau of Enshi Tujia and Miao Autonomous Prefecture,Enshi 445000,Hubei,China
  • Received:2019-11-15 Online:2020-02-25 Published:2020-05-13

Abstract: The total flavonoids were extracted by 60% ethanol-ultrasound from the samples of Ampelopsis grossedentata collected from Qiyueshan mountain (1 500~1 600 m),Lichuan city, Hubei province. The total flavonoids in the young tips of Ampelopsis grossedentata was up to 453.67±11.26 mg/g detected by ultraviolet-visible spectrophotometer at the double-wavelength (λ=208.4 nm,λ=293.2 nm). Three compounds were purified from the organic extract using LC-HR-ESIMS,1D and 2D NMR spectral data analysis. Their structures were identified to be dihydromyricetin(1), myricetin(2),and taxifolin(3). The contents of dihydromyricetin, myricetin and taxifolin were 186.31±10.43, 13.83±1.32 and 14.76±2.26 mg/g respectively detected by HPLC.

Key words: Ampelopsis grossedentata, total flavonoids, dihydromyricetin, myricetin, taxifolin, Lichuan city

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