Abstract: A method for the determination of total flavonoids and mangiferin in mango flesh was established. The total flavonoids in mango flesh were determined by uv-spectrophotometer. Mangiferin was determined by high performance liquid chromatography. The results showed that the total flavonoids had a good linear relationship in the concentration range of 17~520 μg/mL （r=0.999 9）, the average recovery was 99.03%, and the RSD was 1.42% （n=6）. Mangiferin showed a good linear relationship in the concentration range of 4.0~80 μg/mL （r=0.999 9）, with an average recovery of 101.59% and RSD of 1.87% （n=6）. The method developed in this paper was simple, accurate and reproducible. It can be used for the determination of total flavonoids and mangiferin in mango flesh.

Key words: total flavonoids, mangiferin, uv-spectrophotometer, liquid chromatography