Study on extraction technology of total flavonoids from Chinese medicine Excoecaria cochinchinensis Lour.

doi:10.14088/j.cnki.issn0439-8114.2020.14.029

Abstract

Abstract: The total flavonoids of Excoecaria cochinchinensis Lour. were extracted by reflux extraction method. The effects of extraction method, extraction solvent volume fraction, extraction volume and extraction time on the total flavonoid extraction rate of the medicinal materials were investigated by single factor experiment. The orthogonal test was used to optimize the extraction conditions. The best extraction process for a total flavonoid of Excoecaria cochinchinensis Lour. was screened out. The result showed that the optimal extraction process of a total flavonoid of Excoecaria cochinchinensis Lour. was 70% methanol, the ratio of material to liquid was 1∶40（g∶mL）, and the reflux extraction time was 1.0 h. This method can effectively determine the content of total flavonoids in Excoecaria cochinchinensis Lour. The repeatability was good, the method was stable and feasible.

Key words: Excoecaria cochinchinensis Lour., total flavonoids, extraction process

CLC Number:

R282

HAN Qian, WEI Jiang-cun, CHEN Yong, LU Jing-rong, LI Jin-zhou, CHEN Zi-jun. Study on extraction technology of total flavonoids from Chinese medicine Excoecaria cochinchinensis Lour.[J]. HUBEI AGRICULTURAL SCIENCES, 2020, 59(14): 145-148.

References

[1] 云南省药物研究所. 云南药用植物名录[M]. 昆明:云南省药物研究所,1975.127.
[2] 中国科学院中国植物志编辑委员会. 中国植物志( 第44卷第3分册)[M]. 北京: 科学出版社,1997.
[3] 杨延明,芦翠乔,蒋武生,等. 红背桂花的组织培养及植株再生[J]. 河南农业科学,1990(8):25,34.
[4] 中华本草编委会. 中华本草(第12卷)[M]. 上海:上海科学技术出版社,1999.
[5] GIANG P M,SON P T,MATSUNAMI K,et al.New megastigmane glucosides from Excoecaria cochinchinensis LOUR. var. cochinchinensis[J]. Chemical & pharmaceutical bulletin,2005,53(12):1600-1603.
[6] 李子燕,杨靖华,汪云松,等. 红背桂花化学成分研究[J]. 中草药,2006(6):826-829.
[7] 陈兵祥,王小玲. 红背桂花的化学成分研究[J]. 药学研究,2015,34(3):147-149.
[8] 王业玲,黎平,郭凯静,等. 红背桂花化学成分研究[J]. 天然产物研究与开发,2014,26(1):47-49.
[9] 汪云松,黄荣,张洪彬,等. 红背桂花化学成份研究[J]. 热带亚热带植物学报,2009,17(2):156-159.
[10] 王硕,周小雷,袁经权,等. 红背桂药材的质量标准研究[J]. 中国药房,2013,24(27):2542-2545.
[11] 李萌茹,周玉枝,杜冠华,等. 中药黄酮类化合物抗衰老作用及其机制研究进展[J/OL]. 药学学报,http://kns.cnki.net/kcms/detail/11.2163.R.20190628.1634.017.html,2019.
[12] 曹剑锋,刘其高,蒋小飞,等. 阴地蕨总黄酮的提取及抗氧化活性研究[J]. 江苏农业科学,2018,46(21):228-231.
[13] 魏江存,陈勇,谢臻,等. 10批不同产地六棱菊中咖啡酸的工艺优化与含量测定[J]. 中国药房,2017,28(34):4792-4795.
[14] 李思阳,孔庆新,庄润泽,等. 香椿子中总黄酮提取工艺的优化和比较[J]. 中成药,2017,39(10):2178-2182.
[15] 魏江存,陈勇,谢臻,等. 六棱菊薄层鉴别和绿原酸的提取工艺及其含量测定[J]. 时珍国医国药,2017,28(11):2611-2614.
[16] 陈勇,李金洲,魏江存,等. 壮药一匹绸薄层鉴别和咖啡酸提取工艺及含量测定[J]. 吉林中医药,2019,39(1):92-97.