Universality validation of molecular markers for sex identification in Idesia polycarpa Maxim.

doi:10.14088/j.cnki.issn0439-8114.2023.10.019

Abstract

Abstract: Using 120 samples of Idesia polycarpa with known sex, the universality of the two reported molecular markers UBC841 and STZ was tested for sex determination of Idesia polycarpa. Meanwhile, a large number of inter-simple sequence repeat （ISSR） and sequence related amplified polymorphisms（SRAP） markers were analyzed for their polymorphism in male and female plants of Idesia polycarpa. The results showed that UBC841 could detect polymorphic bands in 24 samples of Idesia polycarpa, but no gender-specific bands were detected. STZ could amplify the target band in some samples, but did not show the sex specificity of male and female bands. These two groups of markers did not show good universality, and the accuracy of gender discrimination was low. In addition, 26 ISSR primers and 780 pairs of SRAP primers were screened, but no sex-specific markers were obtained, indicating that it was difficult to screen stable and reliable sex-specific markers in Idesia polycarpa by using universal molecular markers ISSR and SRAP.

Key words: Idesia polycarpa Maxim., sex identification, inter-simple sequence repeat（ISSR）, sequence related amplified polymorphism（SRAP）, universality validation

CLC Number:

Q945.6⁺1

YAN Li, TONG Zhu, HE Xiu-juan, XIAO Cui, WANG Ze-qiong, YUAN Long-yi, SUN Zhong-hai, QIU Wen-ming. Universality validation of molecular markers for sex identification in Idesia polycarpa Maxim.[J]. HUBEI AGRICULTURAL SCIENCES, 2023, 62(10): 109-114.

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