Isolation and identification of a strain of Aviobacterium paragallinarum type A

doi:10.14088/j.cnki.issn0439-8114.2024.11.023

Abstract

Abstract: The bacteria were isolated from diseased materials with swollen heads and swollen faces in a large-scale chicken farm in Hubei province. The molecular biology, biochemical characteristics, serotype and drug resistance of the isolated strain were identified, and the genome sequence of the isolated strain was determined. The pathogenicity of the strain was verified through animal regression experiments. Results showed that white round, smooth edges, translucent dew-like colonies were isolated from the samples. The isolated strain was gram-negative bacterium, which showed a typical “satellite” pattern in satellite tests. The 16S rRNA sequence of isolated strain was highly homologous to reported sequence of Avibacterium paragallinarum. Its serotype was identified as type A. And the results of drug susceptibility tests showed that the isolate strain was resistant to trimethoprim, lincomycin and amikacin, while sensitive to various antibiotics such as doxycycline, spectinomycin. Animal regression experiments showed that the isolated strain caused typical swollen head and face symptoms in chicks. Pathological sections showed the strain caused significant lesions in the lungs, liver and spleen of chicks. The whole-genome sequence of isolated strain showed that it had 53 drug resistance genes and 121 virulence genes.

Key words: Avibacterium paragallinarum, biochemical characteristics, pathogenicity, genome

CLC Number:

S852.61

YIN Meng-yu, LI Feng, GUO Yun-qing, ZHANG Teng-fei, ZHANG Wen-ting, HU Qiao, LU Qin, ZHAI Xin-guo, LUO Qing-ping. Isolation and identification of a strain of Aviobacterium paragallinarum type A[J]. HUBEI AGRICULTURAL SCIENCES, 2024, 63(11): 134-140.

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