Antibacterial mechanism of Forsythia suspensa aqueous extract against Avibacterium paragallinarum

doi:10.14088/j.cnki.issn0439-8114.2026.01.022

Abstract

Abstract: Avibacterium paragallinarum causes infectious coryza, which leads to decreased egg production in layers and growth retardation in broilers, resulting in significant economic losses to the poultry industry. With the emergence of multidrug resistance and the implementation of antibiotic-restriction policies, there is an urgent need to develop green and effective antibiotic alternatives. Forsythia suspensa（F. suspensa）possesses anti-inflammatory and anti-infective properties. The content of forsythin in the aqueous extract of F. suspensa was determined using High-performance liquid chromatography (HPLC). The antibacterial effects of the extract against Apg were evaluated, including its impact on bacterial growth, biofilm formation, adhesion and invasion capabilities, thereby preliminarily elucidating its antibacterial mechanism. The results showed that the forsythin content in the aqueous extract was （1.67±0.03）%. The minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) of the extract against Apg were both 7.81 mg/mL. The extract significantly inhibited bacterial growth, biofilm formation, and adhesion-invasion capabilities in a dose-dependent manner. These findings demonstrated that the aqueous extract of F. suspensa exhibited potent inhibitory effects against Apg in vitro, providing a potential strategy for developing eco-friendly agents to control Apg infections.

Key words: Forsythia suspensa aqueous extract, Avibacterium paragallinarum, forsythin, High-performance liquid chromatography, antibacterial mechanism

CLC Number:

S859.3

KANG Hong-kai, GUO Yun-qing, ZHANG Teng-fei, ZHANG Wen-ting, HU Qiao, LU Qin, ZHANG Rong-rong, ZHAI Xin-guo, LUO Qing-ping. Antibacterial mechanism of Forsythia suspensa aqueous extract against Avibacterium paragallinarum[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(1): 131-136.

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