湖北农业科学 ›› 2020, Vol. 59 ›› Issue (15): 114-117.doi: 10.14088/j.cnki.issn0439-8114.2020.15.022

• 贮藏·加工 • 上一篇    下一篇

正交法优化黄精多糖的提取工艺

王毅1,2, 穆麒麟1,2, 田小海1,2, 武芸3   

  1. 1.长江大学农学院,湖北 荆州 434025;
    2.长江大学主要粮食作物产业化湖北省协同创新中心,湖北 荆州 434025;
    3.湖北民族学院生物科学与技术学院,湖北 恩施 445000
  • 收稿日期:2020-06-22 出版日期:2020-08-10 发布日期:2020-09-24
  • 通讯作者: 田小海(1961-),男,湖北仙桃人,教授,博士生导师,博士,主要从事作物高产原理、作物逆境及耐逆技术方向研究,(电子信箱)xiaohait@sina.com。
  • 作者简介:王 毅(1995-),男,湖北恩施人,在读硕士研究生,研究方向为作物栽培技术,(电话)18727384350(电子信箱)457969945@qq.com。
  • 基金资助:
    国家重点研发计划项目(SQ2018YFD030224); 长江大学“大学生创新创业训练计划”项目(2017177)

Optimization of extraction technology of polysaccharide in Polygonatum sibiricum by orthogonal method

WANG Yi1,2, MU Qi-lin1,2, TIAN Xiao-hai1,2, WU Yun3   

  1. 1. College of Agriculture,Yangtze University,Jingzhou 434025,Hubei,China;
    2. Hubei Collaborative Innovation Center for Grain Industry,Yangtze University,Jingzhou 434025,Hubei,China;
    3. School of Biological Science and Technology,Hubei University for Nationalities,Enshi 445000,Hubei,China
  • Received:2020-06-22 Online:2020-08-10 Published:2020-09-24

摘要: 以恩施市药用经济植物黄精(Polygonatum sibiricum)地上的茎和叶为试验材料,水为提取溶剂分别对黄精的茎和叶进行试验,得到优化后的提取工艺。研究发现,在料液比为1∶20(g∶mL),提取时间为2 h,温度为80 ℃时,黄精茎多糖的提取率为19.87%,黄精叶多糖的提取率为17.13%。结果表明,黄精茎中的多糖含量高于叶,但总体来看黄精的茎和叶中的多糖含量还是较高的,工业生产可以考虑进一步利用。

关键词: 黄精(Polygonatum sibiricum), 多糖, 提取工艺, 正交法

Abstract: The stems and leaves of medicinal economic plant Polygonatum sibiricum in Enshi city were used as test materials, and water was used as the extraction solvent to test the stems and leaves of Polygonatum sibiricum respectively, the optimized extraction process was obtained. The study found that when the ratio of material to liquid was 1∶20 (g∶mL), the extraction time was 2 h and the temperature was 80 ℃, the yield of polysaccharide from Polygonatum sibiricum stems was 19.87%, the yield of polysaccharides from Polygonatum sibiricum leaf was 17.13%. The results show that the polysaccharide content in the stems of Polygonatum sibiricum are higher than that in leaves, but in general, the polysaccharide content in stems and leaves of Polygonatum sibiricum are still relatively high, and industrial production can be considered for further utilization.

Key words: Polygonatum sibiricum, polysaccharide, extraction process, orthogonal method

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