湖北农业科学 ›› 2021, Vol. 60 ›› Issue (8): 114-117.doi: 10.14088/j.cnki.issn0439-8114.2021.08.023

• 水产养殖 • 上一篇    下一篇

鲤仔鱼耳石上锶标记的持久性评价

邱晨1, 姜涛2, 陈修报2, 刘洪波2, 杨健1,2   

  1. 1.南京农业大学无锡渔业学院,江苏 无锡 214081;
    2.中国水产科学研究院淡水渔业研究中心院长江中下游渔业生态环境评价与资源养护重点实验室,江苏 无锡 214081
  • 收稿日期:2020-08-14 发布日期:2021-05-07
  • 通讯作者: 杨 健,男,研究员,博士,主要从事渔业生态环境监测与保护研究,(电话)051085557823(电子信箱)jiany@ffrc.cn。
  • 作者简介:邱 晨(1981-),女,江西上饶人,硕士,主要从事渔业生态环境监测与保护研究,(电话)18375681942(电子信箱)657465328@qq.com
  • 基金资助:
    中央级公益性科研院所基本科研业务费专项(2019JBFM06号); 农业农村部农业财政专项(CJDC-2017-22号)

Persistence evaluation of strontium marking on otoliths in larvae common carp (Cyprinus carpio

QIU Chen1, JIANG Tao2, CHEN Xiu-bao2, LIU Hong-bo2, YANG Jian1,2   

  1. 1. Wuxi Fisheries College,Nanjing Agricultural University,Wuxi 214081, Jinagsu,China;
    2. Key Laboratory of Fishery Ecological Environment Assessment and Research Conservation in Middle and Lower Reaches of the Yangtze River,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,Jinagsu,China
  • Received:2020-08-14 Published:2021-05-07

摘要: 为了探索Sr标记在鱼类耳石上留存的持久性,将1尾鲤(Cyprinus carpio)仔鱼在10 mg/L六水氯化锶(SrCl2·6H2O)溶液中浸泡标记2 d后,让其回到正常水体中恢复续养607 d(即20个月)时采样,再利用电子探针X射线微化学分析(EPMA)技术来确认其耳石上的Sr标记是否能长期存在。EPMA面分布和定量线分布结果均显示,即使在浸泡标记完成后640 d时,该鲤矢耳石、星耳石和微耳石上均能检测到明显的Sr标记区(但星耳石需要通过横截面来测定)。该标记方法在鲤增殖放流标记和效果评价中具有持久性。

关键词: 鲤(Cyprinus carpio), 锶, 标记, 耳石, 持久性

Abstract: In order to investigate the persistence of Sr markings on otoliths, the larvae common carp (Cyprinus carpio) were marked in this study, using a single concentration of high Sr solution for 2 d immersion. A recognizable Sr marking region could be detected in the otolith, after a long period of recovery, using electron probe X ray micro chemical analysis(EPMA) technique. The results of mapping analysis of Sr content and the fluctuation of Sr/Ca concentration ratios along line transect from the core (0 μm) to the edge in otoliths showed that even after 607 d (i.e., 20 months) of recovery culture, a ring of red Sr marking region was still kept in the otolith. The Sr mark of otolith was firstly confirmed existence for quite a long time at least 20 months after exogenous Sr marking, suggesting that this method exactly have a strong stability, reliability, durability and application potential in the large-scale marking for fish proliferating and releasing and evaluation of corresponding effect.

Key words: common carp (Cyprinus carpio), strontium (Sr), marking, otolith, persistence

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