水稻雄性不育突变体Osps-2基因的遗传定位

doi:10.14088/j.cnki.issn0439-8114.2025.09.035

湖北农业科学 ›› 2025, Vol. 64 ›› Issue (9): 229-237.doi: 10.14088/j.cnki.issn0439-8114.2025.09.035

水稻雄性不育突变体Osps-2基因的遗传定位

甘甜^1,2, 蔡凯², 涂泽琰², 汪超^1,2

1.湖北工业大学生命科学与健康工程学院,武汉 430070;
2.武汉一元堂生物科技股份有限公司,武汉 430070

收稿日期:2025-02-18 出版日期:2025-09-25 发布日期:2025-10-28
通讯作者: 汪超（1978-）,男,湖北武汉人,教授,博士,主要从事食品发酵及酿造工程研究,（电话）18986138612。
作者简介:甘甜（1988-）,女,湖北武汉人,硕士,主要从事食品工程研究,（电话）13720246430（电子信箱）418034364@qq.com。
基金资助:
湖北省技术创新计划项目（2024BBB034）

Genetic mapping of the Osps-2 gene responsible for male sterility mutant in rice

GAN Tian^1,2, CAI Kai², TU Ze-yan², WANG Chao^1,2

1. School of Life and Health Sciences, Hubei University of Technology, Wuhan 430070, China;
2. Wuhan Yiyuantang Biotechnology Co., Ltd., Wuhan 430070, China

Received:2025-02-18 Published:2025-09-25 Online:2025-10-28

摘要/Abstract

摘要： 为解析水稻（Oryza sativa L.）雄性不育的分子机制及其在作物遗传改良中的应用潜力,利用EMS（甲基磺酸乙酯）诱变技术获得不育突变体,命名为Osps-2。与野生型日本晴相比,Osps-2突变体的营养生长未表现出明显差异,但成熟后无种子。形态学观察显示,Osps-2突变体在成熟期的花药中几乎未观察到正常花粉,表明其不育表型可能源于花粉发育异常。细胞学分析发现,小孢子从四分体释放后,野生型日本晴的小孢子能够顺利完成后续发育过程,最终形成成熟花粉;Osps-2突变体的小孢子从四分体释放后出现降解现象,伴随花粉囊逐渐萎缩,无花粉形成。通过F₂分离群体中不育单株的筛选及分子标记遗传分析,成功将Osps-2基因定位于水稻10号染色体18 809~20 269 kb区间。

关键词: 水稻（Oryza sativa L.）, 雄性不育, 突变体, Osps-2基因, 遗传定位

Abstract: To elucidate the molecular mechanism of male sterility in rice (Oryza sativa L.) and its potential application in crop genetic improvement, a sterile mutant was obtained using EMS (ethyl methanesulfonate) mutagenesis and named Osps-2. Compared with the wild-type Nipponbare, the Osps-2 mutant did not show significant differences in vegetative growth but produced no seeds at maturity.Morphological observation revealed that almost no normal pollen was observed in the anthers of the Osps-2 mutant at the mature stage, indicating that its sterility phenotype might originate from abnormal pollen development. Cytological analysis found that after release from the tetrad, the microspores of wild-type Nipponbare could successfully complete subsequent development and eventually form mature pollen. In contrast, the microspores of the Osps-2 mutant degraded after release from the tetrad, accompanied by gradual shrinkage of the pollen sacs and no pollen formation. Through the screening of sterile individuals in the F2 segregation population and genetic analysis with molecular markers, the Osps-2 gene was successfully mapped to a region between 18 809 kb and 20 269 kb on chromosome 10 of rice.

Key words: rice (Oryza sativa L.), male sterility, mutant, Osps-2 gene, genetic mapping

中图分类号:

S511

甘甜, 蔡凯, 涂泽琰, 汪超. 水稻雄性不育突变体Osps-2基因的遗传定位[J]. 湖北农业科学, 2025, 64(9): 229-237.

GAN Tian, CAI Kai, TU Ze-yan, WANG Chao. Genetic mapping of the Osps-2 gene responsible for male sterility mutant in rice[J]. HUBEI AGRICULTURAL SCIENCES, 2025, 64(9): 229-237.

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水稻雄性不育突变体Osps-2基因的遗传定位

Genetic mapping of the Osps-2 gene responsible for male sterility mutant in rice

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