外源dsRNA介导的RNA干扰靶向沉默CP基因抑制烟草马铃薯Y病毒侵染

doi:10.14088/j.cnki.issn0439-8114.2026.02.015

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 101-106.doi: 10.14088/j.cnki.issn0439-8114.2026.02.015

外源dsRNA介导的RNA干扰靶向沉默CP基因抑制烟草马铃薯Y病毒侵染

许汝冰¹, 罗朝鹏², 黎妍妍¹, 刘俊霞², 杨军², 覃耀¹

1.湖北省烟草科学研究院,武汉 430030;
2.中国烟草总公司郑州烟草研究院,郑州 450001

收稿日期:2025-10-22 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 覃耀(1993-),男,湖北恩施人,博士,主要从事烟草绿色防控技术研究,(电子信箱)qinyao1246282025@163.com。
作者简介:许汝冰(1979-),男,湖北长阳人,高级农艺师,硕士,主要从事烟草绿色防控技术研究,(电子信箱)kubingbing@163.com。
基金资助:
湖北省烟草公司重点项目(027Y2019-002)

Exogenous dsRNA-mediated RNA interference suppresses potato virus Y (PVY) infection in tobacco by targeting and silencing the CP gene

XU Ru-bing¹, LUO Zhao-peng², LI Yan-yan¹, LIU Jun-xia², YANG Jun², QIN Yao¹

1. Tobacco Research Institute of Hubei Province, Wuhan 430030, China;
2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China

Received:2025-10-22 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 为评价外源双链RNA(dsRNA)介导的RNA干扰(RNA interference,RNAi)对烟草马铃薯Y病毒(Potato virus Y,PVY)侵染的抑制效果,克隆了PVY的衣壳蛋白(CP)基因,通过序列分析评估并设计了靶向该基因的dsRNA片段。体外合成制备了长度为474 bp的PVY CP基因特异性dsRNA,并将其与PVY共接种于烟草植株。通过实时荧光定量PCR（qRT-PCR）检测接种叶片中CP基因的mRNA水平,观察病毒症状发展,并统计发病率和病情指数,系统评价该dsRNA对PVY的防治效果。结果表明,外源喷施PVY CP基因dsRNA能够显著降低接种叶片中PVY CP基因的表达量,并有效减轻病毒引起的症状,在盆栽试验中防治效果达50.58%。

关键词: 烟草马铃薯Y病毒(PVY), 双链RNA(dsRNA), RNA干扰(RNAi), 衣壳蛋白(CP), 生物防治

Abstract: To evaluate the inhibitory effect of exogenous double-stranded RNA (dsRNA)-mediated RNA interference (RNAi) on potato virus Y (PVY) infection in tobacco, the coat protein (CP) gene of PVY was cloned. Subsequently, a dsRNA fragment targeting this gene was designed based on sequence analysis. A 474-bp dsRNA targeting the PVY CP gene was synthesized in vitro and applied together with PVY onto tobacco plants. The relative mRNA level of the CP gene in the inoculated leaves was determined by quantitative real-time PCR (qRT-PCR), while viral symptom development was observed over time. Both the disease incidence and disease index were recorded to comprehensively evaluate the control efficacy of the dsRNA treatment against PVY. The results showed that exogenous application of PVY CP gene targeting dsRNA significantly suppressed the expression of the PVY CP gene in the inoculated leaves and effectively alleviated virus-induced symptoms, resulting in a control efficacy of 50.58% in pot trials.

Key words: potato virus Y (PVY), double-stranded RNA (dsRNA), RNA interference (RNAi), coat protein (CP), biocontrol

中图分类号:

许汝冰, 罗朝鹏, 黎妍妍, 刘俊霞, 杨军, 覃耀. 外源dsRNA介导的RNA干扰靶向沉默CP基因抑制烟草马铃薯Y病毒侵染[J]. 湖北农业科学, 2026, 65(2): 101-106.

XU Ru-bing, LUO Zhao-peng, LI Yan-yan, LIU Jun-xia, YANG Jun, QIN Yao. Exogenous dsRNA-mediated RNA interference suppresses potato virus Y (PVY) infection in tobacco by targeting and silencing the CP gene[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 101-106.

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外源dsRNA介导的RNA干扰靶向沉默CP基因抑制烟草马铃薯Y病毒侵染

Exogenous dsRNA-mediated RNA interference suppresses potato virus Y (PVY) infection in tobacco by targeting and silencing the CP gene

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