烟草疫霉抗双炔酰菌胺突变株的生物学特性

doi:10.14088/j.cnki.issn0439-8114.2026.02.018

湖北农业科学 ›› 2026, Vol. 65 ›› Issue (2): 120-125.doi: 10.14088/j.cnki.issn0439-8114.2026.02.018

烟草疫霉抗双炔酰菌胺突变株的生物学特性

彭宇¹, 程佳雯², 李彩斌¹, 马黔¹, 马玉玺¹, 何轶¹, 吴学巧¹, 周文亮³, 邓建强⁴, 齐永霞²

1.贵州省烟草公司毕节市公司,贵州毕节 551700;
2.安徽农业大学植物保护学院,合肥 230036;
3.广西壮族自治区烟草公司百色市公司,广西百色 533000;
4.湖北省烟草公司恩施州公司,湖北恩施 445000

收稿日期:2025-08-28 出版日期:2026-03-04 发布日期:2026-03-04
通讯作者: 齐永霞,女,副教授,主要从事植物病害流行及综合治理研究,(电话)13514997692(电子信箱)superpowerqyx@163.com。
作者简介:彭宇(1972-),男,贵州毕节人,高级农艺师,硕士,主要从事烟草绿色生产与管理,(电话)18908591199(电子信箱)pengyu215@msn.com。
基金资助:
中国烟草总公司重点研发项目(110202202014); 中国烟草总公司广西壮族自治区公司科技计划项目(202245000027147)

Biological characteristics of Phytophthora nicotianae resistant mutants to mandipropamid

PENG Yu¹, CHENG Jia-wen², LI Cai-bin¹, MA Qian¹, MA Yu-xi¹, HE Yi¹, WU Xue-qiao¹, ZHOU Wen-liang³, DENG Jian-qiang⁴, QI Yong-xia²

1. Bijie Branch of Guizhou Provincial Tobacco Company, Bijie 551700, Guizhou, China;
2. School of Plant Protection, Anhui Agricultural University, Hefei 230036, China;
3. Baise Branch of Guangxi Zhuang Autonomous Region Tobacco Company, Baise 533000, Guangxi, China;
4. Enshi Branch of Hubei Provincial Tobacco Company, Enshi 445000, Hubei, China

Received:2025-08-28 Published:2026-03-04 Online:2026-03-04

摘要/Abstract

摘要： 为了明确烟草疫霉抗双炔酰菌胺突变株的生物学特性、抗药性遗传稳定性及突变株的致病性,以烟草疫霉菌株YCHJ为供试菌株,采用紫外线诱导的方法获得烟草疫霉抗双炔酰菌胺突变株;通过菌落形态、菌丝生长速率、游动孢子囊产生量及形态、游动孢子萌发能力等特征比较突变株与亲本菌株的生物学特性;采用菌丝生长速率法和温室盆栽试验分别检测了突变株的遗传稳定性和致病性。结果显示,菌丝块紫外诱导获得的3株突变株YCHJ2-12、YCHJ5-7和YCHJ5-16对双炔酰菌胺的抗性水平分别是62.99、47.06和10.11。游动孢子紫外诱导获得的2株突变菌株YCHJZ2-2和YCHJZ4-13的抗性水平分别是54.79和17.75。生物学特性研究结果显示,突变株和亲本菌株在菌丝形态及游动孢子囊形态上无明显差异,但在菌丝生长速率、孢子囊大小及乳突高度等方面存在差异。突变株YCHJ2-12和YCHJZ2-2菌丝的平均生长速率显著高于亲本菌株YCHJ;突变株YCHJZ4-13产生的游动孢子囊长度、宽度及乳突高度均显著高于亲本菌株YCHJ。此外,突变株YCHJZ4-13、YCHJ5-16和YCHJZ2-2的游动孢子囊产生量显著高于亲本菌株YCHJ,但突变株的游动孢子萌发率及致病性比亲本菌株YCHJ低。菌株YCHJZ4-13和YCHJ5-16的抗药性不能稳定遗传,其他菌株的抗药性随着继代培养时间的延长呈下降趋势。综上所述,烟草疫霉对双炔酰菌胺的抗性风险为低到中等。

关键词: 烟草黑胫病, 双炔酰菌胺, 抗性突变体, 生物学特性

Abstract: In order to clarify the biological characteristics, genetic stability of drug resistance, and pathogenicity of mandipropamid-resistant strains of Phytophthora nicotianae, the P. nicotianae strain YCHJ was used as the tested strain. Mandipropamid-resistant strains of P. nicotianae were obtained by UV-irradiating. The biological characteristics of mutant strains were compared with those of their parent strain such as colony morphology, hyphal growth rate, zoosporangia morphology and production, and germination ability of zoospores. The genetic stability and pathogenicity of the mutant strains were assessed by the mycelial growth rate method and greenhouse pot trials, respectively. The results showed that the resistance levels of the mutant strains YCHJ2-12, YCHJ5-7 and YCHJ5-16 induced by mycelial UV-irradiating were 62.99, 47.06 and 10.11, respectively. The resistance levels of the mutant strains YCHJZ2-2 and YCHJZ4-13 induced by UV-irradiating of zoospores were 54.79 and 17.75, respectively. The results of biological characteristics showed that there was no significant difference in hyphal morphology and zoosporangia morphology between the mutant strains and the parent strain, but there were differences in the mycelial growth rate, the size of zoosporangia and the height of papillae. The average mycelial growth rate of YCHJ2-12 and YCHJZ2-2 was significantly higher than that of the parental strain YCHJ. The length, width and papillae height of the zoosporangia produced by YCHJZ4-13 were significantly higher than those of YCHJ. In addition, the zoosporangia production of YCHJZ4-13, YCHJ5-16 and YCHJZ2-2 was significantly higher than that of YCHJ, but the germination rate of zoospores and pathogenicity of the mutant strains were lower than those of YCHJ. The resistance of YCHJZ4-13 and YCHJ5-16 to mandipropamid couldn’t be stably inherited, and the resistance of other strains decreased with the increase of cultivation generations. Based on these, it was speculated that the resistance risk of P. nicotianae to mandipropamid was low to moderate.

Key words: tobacco black shank, mandipropamid, resistant mutant, biological characteristics

中图分类号:

S572

彭宇, 程佳雯, 李彩斌, 马黔, 马玉玺, 何轶, 吴学巧, 周文亮, 邓建强, 齐永霞. 烟草疫霉抗双炔酰菌胺突变株的生物学特性[J]. 湖北农业科学, 2026, 65(2): 120-125.

PENG Yu, CHENG Jia-wen, LI Cai-bin, MA Qian, MA Yu-xi, HE Yi, WU Xue-qiao, ZHOU Wen-liang, DENG Jian-qiang, QI Yong-xia. Biological characteristics of Phytophthora nicotianae resistant mutants to mandipropamid[J]. HUBEI AGRICULTURAL SCIENCES, 2026, 65(2): 120-125.

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烟草疫霉抗双炔酰菌胺突变株的生物学特性

Biological characteristics of Phytophthora nicotianae resistant mutants to mandipropamid

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